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Promoter glutamicum

The further engineering of the promoter and the generation of the respective strain without any vector sequences is outlined in Fig. 11. Based on known sequences of strong promoters of C. glutamicum directed mutations were introduced, which among others resulted in promoter MC 20 with nearly four-fold... [Pg.25]

Figure 63 Maps of plasmid vectors for C glutamicum (adapted from [100]) (a) C glutamicum/E. coli shuttle promoter-probe vector pET2 and (b) C. glutamicum/E. coli shuttle expression vector pVWExl. Dark areas indicate regions coming from plasmids of Corynebacteria (pBLI, pCGI, and pGAI, respectively). Arro s inside the map... Figure 63 Maps of plasmid vectors for C glutamicum (adapted from [100]) (a) C glutamicum/E. coli shuttle promoter-probe vector pET2 and (b) C. glutamicum/E. coli shuttle expression vector pVWExl. Dark areas indicate regions coming from plasmids of Corynebacteria (pBLI, pCGI, and pGAI, respectively). Arro s inside the map...
Release of the genome sequence of C. glutamicum promoted the development of global analysis of transcription. First microarrays were designed as metabolic... [Pg.197]

Patek, M. and Nesvera, J. (2013) Promoters and plasmid vectors of Corynehacterium glutamicum in Corynehacterium glutamicum - Biology and Biotechnology (eds H. Yukawa... [Pg.209]

Lezyk, M.J., Solem, C., and Jensen, P.R. (2014) Synthetic promoter libraries for Corynehacterium glutamicum. [Pg.209]

L. M. (2006) Characterization and use of catabolite-repressed promoters from gluconate genes in Corynebacterium glutamicum. J. Bacterial, 188 (2),... [Pg.210]

M., Zemanova, M., Nesvera, J., and Patek, M. (2007) Plasmid vectors for testing in vivo promoter activities in Corynebacterium glutamicum and Rhodococcus erythropolis. Cum Microbiol, 55 (3), 234-239. [Pg.210]

Analysis of Corynebacterium glutamicum promoters and their applications. Subcell. Biochem., 64, 203—221. [Pg.211]

Ben-Samoun, K., Leblon, G, and Reyes, O. (1999) Positively regulated expression of the Escherichia coli araBAD promoter in Corynebacterium glutamicum. FEMS Microbiol. Lett., 174 (1), 125-130. [Pg.212]

Okibe, N., Suzuki, N., Inui, M., and Yukawa, H. (2010) Isolation, evaluation and use of two strong, carbon source-inducible promoters from Corynebacterium glutamicum. Lett Appl. Microbiol, 50 (2), 173-180. [Pg.212]

The second system is composed of the RolR regulator and the PAS promoter fused with the operator (rolO) from the Corynebacterium glutamicum resorcinol catabolic operon. This promoter has the low basal level of transcription and can be induced by resorcinol. In summary, there are significant numbers of well-characterized synthetic and inducible promoters to control the expression of gene and gene clusters in streptomycetes [81]. [Pg.495]

Tateno T, Fukuda H, Kondo A. (2007b). Direct production of L-lysine from raw com starch by Corynebacterium glutamicum secreting Streptococcus bovis a-amylase using cspB promoter and signal sequence. Appl Microbiol Biotechnol, 77, 533-541. [Pg.379]

Rytter JV, Helmark S, Solem C, Lezyk MJ, Jensen PR (manuscript in preparation) Synthetic promoter libraries for metabolic engineering of Corynebacterium glutamicum Siegele DA, Hu JC (1997) Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populations. Proc Natl Acad Sci USA 94 8168-8172... [Pg.200]

Analysis of Corynebacterium glutamicum Promoters and Their Applications... [Pg.203]

Keywords Corynebacterium glutamicum Gene expression Promoter Promoter consensus sequence Sigma factor... [Pg.203]

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