Prokaryotes gene libraries

The construction of a prokaryotic gene library can be achieved by a technique called shotgun cloning (Fig. 24.4). This involves the purification and partial digestion of the genomic (chromosomal) DNA... 

Park KS, Jang YS, Lee H, Kim JS (2005) Phenotypic alteration and target gene identification using combinatorial libraries of zinc finger proteins in prokaryotic cells. J Bacteriol 187 5496-5499... 

Before we study how genomic libraries are made we first need to understand the differences between the genetic organization in eukaryotic and prokaryotic cells. Bacterial genes are uninterrupted sequences of... 

DNA (section 2.4.1) than eukaryotic RNA (section 2.4.2). The result will be the construction of a collection of cloned DNA fragments propagated in bacteria that is called the genomic library. This library should contain representatives of every sequence in the chromosome of a prokaryotic cell and every expressed gene in the case of a eukaryotic cell. The final step consists of the screening of every recombinant clone to identify the required gene(s). 

Now that we have briefly introduced some of the most important tools and techniques of molecularbiology, we can return to the isolation, cloningand identification of genes ofinterest from within prokaryotic genomic DNA or eukaryotic cDNA library sequences. There are two main approaches for this. 

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