Processing protein-nanoparticle composites using extrusion

Processing protein-nanoparticle composites using extrusion 

For the protein nanocomposite formulation, 400 g of water and 400 g of glycerol were also blended in a large beaker with a mechanical stirring device for two minutes, prior to the addition of 60 g of Cloisite Na. For the untreated system the mixture was then eombined drip-wise with 1200 g of the soy protein using a ten-litre laboratory seale high-speed mixer (HSM-10) for five minutes prior to extrusion similar to the neat protein film. For the ultrasonically treated system the mixture was treated with a point souree ultrasonic device (Branson sonifier model 250 W cell disruptor) for one hour in an iee-eooled environment (as outlined in section 11.2). This mixture was then eombined drip-wise to 1200 g of the soy protein using a ten-litre laboratory seale high-speed mixer (HSM-10) for five minutes prior to extrusion similar to the neat protein film. 

4 Microstructure and mechanical properties of protein-nanoparticle composites 

The main tools used for assessing the dispersion of elays in these nanocomposites were WAXS and transmission electron microscopy (TEM). The WAXS measurements were performed using a Briiker D8 Diffraetometer as described in section 11.2. Monitoring the d-ooi spacing corresponding to the 

9 WAXS diffractograms of the neat protein/glycerol, neat Cloisite Na and the resulting nanocomposite films. 

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Processing protein-nanoparticle composites using extrusion... 

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