Prereplication complex

Nasmyth If you reactivated the kinase, given that the kinase inhibits prereplication complex formation, this would do the job. There are two ways of preventing replication one is not to make the components of the pre-replication complex, the other is to keep the kinase high, which inhibits that formation. This could be an explanation of the Nenopus work. 

The study of replication in yeast ARSs and artificial chromosomes has revealed that initiation of replication requires not only an initiator protein but a complex of six proteins that form an origin recognition complex (ORC).493 4953 This complex, which is essential to initiation of replication, may be joined by additional proteins in a prereplication complex. At least some of the ORC proteins have their homologous counterparts in metazoa, suggesting a highly conserved initiation machinery.4953-0... 

In anaphase and during Cl, a prereplication complex (pre-RC) is formed at replication initiation sites which contains the Cdc6 protein and the MCM proteins, in addition to the constitutively bound origin recognition complex (ORC). Formation of pre-RC is negatively regulated by the C2/M cyclin -CDK complex. The Cdc6 protein and the MDM proteins are removed at the Cl-S transition due to phosphorylation by the S-phase cyclin - CDK complex which allows the onset of DNA replication. The activity of the cyclin-CDK complexes is con-... 

Following activation of an origin and initiation of DNA synthesis, the Cdc6 protein becomes phosphorylated and dissociates from the origin, causing transition of the prereplication complex to a post-replication complex that is no longer able to initiate DNA replication. 

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