DNA replication complexes

Applegren N, Hickey RJ, Kleinschmidt AM, Zhou Q, Coll J, Wills P, Swaby R, Wei Y, Quan JY, Lee MY et al. (1995) Further characterization of the human cell multiprotein DNA replication complex. J Cell Biochem 59 91-107... 

Once the replication fork is established, other proteins begin assembling the functional DNA replication complex. 

Simbulan-Rosenthal CM, Rosenthal DS, Hilz H, Hickey R, Malkas L, Applegren N, Wu Y, Bers G, and Smulson ME (1996) The expression of poly(ADP-ribose) polymerase during differentiation-linked DNA replication reveals that it is a component of the multiprotein DNA replication complex. Biochemistry 35,11622-33. 

Doublie S, Tabor S, Long AM, Richardson CC, Ellenberger T. Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 A resolution. Nature 1998 391 251-258. 

The cyclin A-CDK2 complex has been shown to phosphorylate, among others, the transcription factor E2F1 and components of the DNA replication complex. 

The DNA replication complex requires a variety of proteins to facilitate fork movement. Which answer best describes the minimum set of proteins required for E. coli DNA replication in an vitro reaction ... 

BER, base excision repair DNA-PK, DNA-dependent protein kinase DSB, double strand break MNNG, N-methyl-N -nitro-N-nitrosoguanidine MRC, multiprotein DNA replication complex NER, nucleotide excision repair PAR, poly(ADP-ribose) PARP-1, poly(ADP-ribose) pol)mierase-l PCNA, proliferatii cell nuclear ant en p21, p21 pol 5, DNA pol)mierase 5 SSB, single strand break WRN, Werner syndrome protein. 

Lebel M, Spillare EA, Harris CC et al. The Werner syndrome gene product copurifies with the DNA replication complex and interacts with PCNA and topoisomerase I. J Biol Chem 1999 274 37795-37799. 

Finally a number of gioups recendy came up with an intriguing new aspect of the link between PARP-1 and aging There are a few rare genedc disorders that induce premature aging in humans. One of these examples is Werner syndrome, due to mutation in the WPiNgput. The WRN gene encodes the WRN protein, a member of the RecQ helicase family which comprises an additional exonudease activity. WRN is part of the DNA replication complex and cooperates with several replication- and repair-relat proteins. Recendy, it was shown... 

Fig. 5 Aui.4 n,/streptavidin-labeled DNA replication complexes. Stable replication complexes that were isolated contained a 30-mer, a 21-mer complementary strand, polymerase S (Pol S, 175 kDa), and its auxiliary factor, proliferating cell nuclear antigen (PCNA, a trimer of 90 kDa). A 10-mer extension was put on the 30-mer with two biotinylated bases, which were then labeled with streptavidin with 1.4-nm gold (Nanogold) attached (arrows). Rods are tobacco mosaic virus. Unstained freeze-dried sample. Full width, 256 nm, (Figure produced in collaboration with Paul Fisher, State University of New York at Stony Brook.)...

Handa H, Carter BJ. Adeno-associated vims DNA replication complexes in herpes simplex vims or adenovims-infected cells. J Biol Chem 1979 254 6603-6610. 

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