Preparative chromatography classification

Reversed phase silicas are discussed in depth here as this type of packing materials is often applied in preparative chromatography. According to the classification used above, reversed phase silica is an example of a designed adsorbent. 

Analysis of the purity of each silica gel column fraction and classification of the unknown lipid can be accomplished by thin-layer chromatography on silica gel plates. On a single plate will be spotted (1) a solution of the crude lipid extract from part A, (2) aliquots from each lipid fraction of the column (or recrystallized lipid), and (3) solutions of standard lipids (listed in the Materials section). On a 20 X 20 cm silica gel plate there is room for nine different analyses. Prepare a 1% solution of the crude lipid from part A in chloroform (10 mg/1 mL). If recrystallized lipid is to be... 

A particular paragraph is devoted to some specific requirements for FFF cell sorting, such as sample preparation or separator poisoning. The battery of cell population or cell subpopulation characterization methods will be described with an FFF-dependent classification physical methods compared to functional ones. Finally, experimental correlations between functional and physical cell characteristics lead to the isolation of very specific populations, thereby opening the field of cellulomics. In this report, it is assumed that readers have a basic knowledge of separation sciences, in particular, in chromatography and FFF. 

Another classification of TLC is off-line versus on-line (Nyiredy, 1992). Classical TLC is off-line in that the different steps are performed separately. Overpressured and rotational preparative planar chromatography can be performed on-line by use of an injector, flow-through detector, and fraction collector. 

Sweet potato toxins, 1042 Synthetic dyes, 1001-0132 chromatography of dyes, 1002-1003 classification, application, and colors, 1001-1002 HPTLCofdyes, 1024-1026 identification, analysis, and evaluation, 1002 OPLCofdyes, 1023-0124 preparation of samples and thin layers, 1003-1006... 

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