Microinjection capillary preparation

Figure 10 Enrichment and separation setup for SLM extraction. A - peristaitic pump, B - membrane device with hoiiow fiber instaiied into fused siiica capiiiary, C - iniet on the donor side, D - washing iine used after enrichment, E - microinjection pump to transport the acceptor into ioop F, G - Cis packed capillary column, H - detector window, I - splitter unit, J - high pressure pump. (Adapted from Thordarson E, Palmarsdottir S, Mathiasson L, and Jonsson JA (1996) Sample preparation using a miniaturized supported liquid membrane device connected on-line to packed capillary liquid chromatography. Analytical Chemistry 68 2559-2563.)...

Microinjection pipets are prepared as follows PuU fine glass capillaries with inner filament (Clark Electromedical Instruments, Reading, UK 1.0-mm outside diameter and... 

For microinjection experiments, standard or inverted microscopes are used (e.g., Zeiss Axioskop with transmitted light phase contrast and the mechanical micromanipulator from Leitz (see Fig. 1). The capillary puller is homemade (Fig. 2). The glass tubes (1.4-mm outer and 1.2-mm inner diameters) for preparation of the injection capillaries are obtained from either Schott or Clark Electromedical Instruments. Sterile capillaries can be obtained from Eppendorf. 

Prepare dissection needles. Wear gloves to avoid contaminating the glass needles. Pull each capillary tube into two needles using any program for microinjection needles on a needle puller (the ends are broken off later, so the exact profile of the needle is not critical). 

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