Preparation of Fluorescent Silica Nanoparticles

Various types of fluorescent silica nanopartide have been developed some details are summarized in Table 4.7. Fluorescent dyes are attached to silica nanopartides by several means, as indicated in the table. For example, they can be incorporated 

Inorganic silica nanopartides TEOS Organic silica nanopartides MPMS, MPES, MPDMS, Epos 

Sucdnimidyl esters-fluorescent dye conjugates Maleimide-fluorescent dye conjugates 

Flow cytometry analysis was appHed to fluorescent-tuned TEOS nanopartides containing fluorescein, multifluorescent TEOS nanopartides containing fluores- 

TEOS nanoparticles doped with three types of dye were prepared for fluorescence-resonance energy transfer (FRET) by means of imposition [55]. The dyes fluorescein, 5-carboxyrhodamine 6G (R6G) and 6-carboxy-X-rhodamine (ROX) were used to prepare these TEOS nanoparticles because their spectral features effectively overlap. In the triple-dye-doped TEOS nanoparticles, fluorescein was used as a common donor for R6G and ROX, whereas R6G acted both as an acceptor for fluorescein isothiocyanate (FITG) and as a donor for ROX. To prepare the nanoparticles, the three types of amine-reactive dye molecule were first covalently linked to APS, 5-(and-6)-carboxyfluorescein succinimidyl ester, 5-carboxyrhodamine 6G succinimidyl ester and 6-carboxy-X-rhodamine succinimidyl ester. 

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