Precipitation from glutaraldehyde

Figure 20.7 Glutaraldehyde antibody-enzyme crosslinking procedures usually produce a wide range of high-molecular-weight complexes, some of which may precipitate from solution.

Liposomes containing PE residues can be reacted with glutaraldehyde to form an activated surface possessing reactive aldehyde groups. A 2-step glutaraldehyde reaction strategy is probably best when working with liposomes, since precipitated protein would be difficult to remove from a vesicle suspension. 

For Lot B the acellular pertussis vaccine components are produced from B. pertussis cultures grown in Stainer-Scholte medium modified by the addition of casamino acids and dimethyl-betacyclodextrin. Fimbriae types 2 and 3 are extracted from the bacterial cells and the pertussis toxin, FHA, and PRN are prepared from the supernatant. These proteins are purified by sequential filtration, salt precipitation, ultrafiltration, and chromatography. Pertussis toxin is inactivated with glutaraldehyde and FHA is treated with formaldehyde. The individual antigens are adsorbed separately onto aluminum phosphate. Diphtheria and tetanus, toxoids are individually adsorbed onto aluminum phosphate. The adsorbed diphtheria, tetanus, and acellular pertussis components are combined in a sterile isotonic sodium chloride solution containing 2-phenoxyethanol as preservative. Each dose contains 10 pg of PT, 5 pg of FHA, 3 pg of PRN, 5pg of FIM, as well as 15Lf of diphtheria toxoid and 5.0 Lf of tetanus toxoid. 

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