Potato Dextrose Agar PDA

Poured, sterile soybean casein digest agar (SCDA) petri plates Poured, sterile potato dextrose agar (PDA)... 

G.fujikuroi NRRL-2278 obtained from the US Department of Agriculture, maintained on potato dextrose agar (PDA) slants, and stored at 4°C. 

Each strain was received on a Czapek yeast autolysate agar plate from the culture collection. Spores were transferred to a potato dextrose agar (PDA) (Difco, Detroit, MI) plate and the PDA plates were kept at the optimal temperature for growth (Table 1). After 2 wk the strains on the PDA... 

Test for Yeasts and Molds The plate method is used for testing molds and yeast in semisolids. The procedure is similar to that of the total count test. Instead of SBCDA medium, Sabouraud dextrose agar (SDA) medium or potato dextrose agar (PDA) medium is used. Samples are incubated for five to seven days at 20-25 °C to identify the presence of yeasts and molds. 

Claviceps will grow on various organic substrates. Potato Dextrose Agar (PDA) and Malt Extract Agar (MEA) are the most popular. 

Isolates. Pear leaves with sporulating V. nashicola were collected from orchards in Japan, and conidia from the lesions were suspended in sterile distilled water. Drops of conidial suspensions were placed on water agar plates and incubated at 15 C for 3 to 5 days. Agar blocks containing germinated conidia were individually isolated with a steel needle after microscopic observation and transferred onto potato dextrose agar (PDA) slants. After incubation at 20 C for about two months, the... 

Fungal strains Pyricularia oryzae and Drechslera oryzae (parent), sensitive to edifenphos were maintained on potato dextrose agar (PDA). The edifenphos-resistant mutants were maintained on edifenphos-amended PDA. For the present study, uniformaly 8 and 10-day-old cultures of IK oryzae and . oryzae, respectively, were used throughout the study. 

FIGURE 6.3 (A) Aflatoxin B, production on potato dextrose agar (PDA) media incorporating various... 

The inhibition of A. parasiticus growth by [4] was determined in our laboratory using a modification of the assay reported by Amoldi and coworkers (M) stilbene was dissolved in acetone and appropriate amounts were added to sterile potato dextrose agar (PDA) at 50 C to give concentrations of 10, 20, 50, and 100 ug/ml. The medium was dispersed in 60 mm tissue culture dishes and inoculated with 4 mm PDA plugs of actively growing A. parasiticus cultures. Plates were incubated in the dark at 30 C and colony diameters were measured daily. After three days the percent inhibition of A. parasiticus growth at 10, 20, 50, and 100 ug/ml was 15, 26, 37, and 33%, respectively (unpublish data). 

D. Potato Dextrose Agar (PDA) PDA is commercially available from various supply houses and their distributors. Prepare according to manufacturer s recommendations. Sterilize by autoclaving. 

Microorganisms were enumerated by the spread plate technique (Lorch et al., 1995) using tripticasein soy agar (TSA Becton Dickinson, Mexico) for bacteria, and potato dextrose agar (PDA Becton Dickinson, Mexico) with chloramphenicol (Sigma), 100 mg L, for fungi. 

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