Positive polymerase chain reaction controls

Fig. 9.2 Representative examples of the methylation-specific polymerase chain reaction (MSP) analyses for gene promoter regions. Lanes Lf and M indicate the presence of unmethylated and methylated template, respectively. Placental DNA (PDNA) and in vitro methylated DNA (IMD) served as negative and positive controls, respectively. Water (H) was used to detect contamination. Samples 1, 3, 4, and 7 indicate the presence of a methylated promoter DNA with various degrees of methylation, and samples 2, 5, and 6 represent an unmethylated promoter...

Honeycutt, R., Sobral, B. W and McClelland, M. (1997) Polymerase chain reaction (PCR) detection and quantification using a short PCR product and a synthetic internal positive control. Anal. Biochem. 248, 303-306. 

Haematology DNA damage in peripheral blood mononuclear cells and total hydrophilic antioxidant capacity in the plasma from 19 TB patients and healthy tuberculin test-positive controls were evaluated by single-cell gel electrophoresis and inducible isoform Nitric Oxide Synthase (iNOS) expression measured by quantitative polymerase chain reaction (qPCR). Compared to controls, pulmonary TB patients under treatment presented with increased DNA damage and increased antioxidant capacity, which diminished during treatment. TB patients showed lower iNOS expression, but expression tended to increase during treatment [36 ]. 

Prior work in our lab has demonstrated that an adult mesodermal progenitor cell, specifically human MSCs, seeded in a PEGylated fibrin gel within 48 h in vitro began to form vascular tube-like networks, in contrast to controls of unreactive PEG mixed with fibrinogen or fibrin alone [49]. These tubes stained positive for mature EC specific markers like CD31 and vWF. Real-time polymerase chain reaction... 

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