Porous graphitic carbon HPLC chromatography

In addition to high-performance liquid chromatography (HPLC), the chiral resolution using CMPAs was also carried out by supercritical fluid chromatography (SFC) [91] and capillary electrochromatography (CEC) [92-98]. Salvador et al. [91] used dimethylated /1-cyclodextrin as the mobile phase additive on porous graphite carbon as the solid phase for the chiral resolution of tofizopam, warfarin, a benzoxazine derivative, lorazepam, flurbiprofen, temazepam, chlorthalidone, and methyl phehydantoin by SFC. The authors also studied the effect of the concentration of dimethylated /1-cyclodextrin, the concentration of the mobile phase, the nature of polar modifiers, outlet pressure, and the column temperature on the chiral resolution. 

A wide variety of native and derivatized CyDs are available for use as mobile phase additives in HPLC. In liquid chromatography to study the interaction of CyDs with guest molecules, the information about cydodextrin adsorption on the stationary phase is very substantial. It should be noted that the separation ability of bonded CyDs and CyDs added to the mobile phase in HPLC is not always the same (see below). To assess the adsorption of CyDs on the stationary phase, the chromatographic properties of native and permethylated CyDs applied in RP18 and porous graphitic carbon (PGC) column have been studied [25-29]. On the RP18 column the adsorption of yS-CyD is much stronger than that of a- or y-CyDs. On the PGC, the order of elution is a- < < y-CyD, in accordance with the increase of... 

A further example for the analysis of trace levels of pollutants is the determination of polychlorinated dibenzo-dioxins and polychlorinated dibenzofurans in milk. The method includes gel permeation chromatography, alumina cleanup, and porous graphitized carbon chromatography, followed by analysis by GC/high-resolution MS. Moreover, the potential of porous graphitic carbon as an HPLC adsorbent for the isolation of halogenated aromatic compounds has been demonstrated by the fractionation of polychlorinated biphenyls (PCBs), chlorinated pesticides, polychlorinated dibenzo-p-dioxins (PCDDs), and... 

Another Fmoc-based probe, recently developed, contains benzo-fused analogues of the Fmoc molecule. It can be used to purify peptides by either porous graphitized carbon chromatography (7) or RP-HPLC (8). 

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