Pore structure agarose

The family of agarose-based gels, Sepharose, Sepharose CL, and Sepharose Fast Flow, are bead-formed gels prepared from 2, 4, or 6% agarose solutions. The matrix porosity decreases and rigidity of the bead structure increases with increasing agarose concentrations. The open pore structure and broad... 

Ion-exchange resins are cross-linked polymers which are typically polystyrene, cellulose or agarose based. Polystyrene is hydrophobic in nature and useful for inorganic ions and small molecules while cellulose and agarose are hydrophilic and more useful for the larger, biologically important molecules, e.g. proteins and nucleic acids, which either would be adversely affected by a hydrophobic environment or could not gain access to the small pore structure. 

Gel filtration chromatography uses gel such as cross-linked dextrans, polyacrylamide, and agarose as the stationary phase. The gel contains pores of defined sizes. The smaller molecules penetrate the pore structure to a greater extent and therefore have a longer retention time than the larger molecules. 

Preparation of agarose- [270-272] based cryogels, study of their physicochemical properties and peculiarities of the wide pore structure... 

The surface pore structure of seme chronatogr hic supports is shown in Fig. 2. The agarose material was frozen in isopentane surrounded hy liquid nitrogen and the isopentane sublimed off in a freeze-dryer. One portion was dehydrated with acetone, flushed with liquid CD2 and then dried at the... 

Fig. 5-3. Schematic representation of the pore structure of (A) dextran and (B) agarose. The effect of the agarose crystalline microstructure, hydrogen bonding between the individual polymer chains, on the pore geometry and size is clearly evident.

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