Agarose-based gels

The family of agarose-based gels, Sepharose, Sepharose CL, and Sepharose Fast Flow, are bead-formed gels prepared from 2, 4, or 6% agarose solutions. The matrix porosity decreases and rigidity of the bead structure increases with increasing agarose concentrations. The open pore structure and broad... 

Modern cross-linked agarose matrixes (82) may be yet less charged than other polysaccharide-based packings. A recent report (83) implies that KsEC protein-SDS complexes is insensitive to buffer concentration over the range 0.03 M - 0.1 M on such agarose-based gels. 

Superdex is a composite media of agarose and dextran. The base particle is formed of cross-linked agarose to which dextran is then covalently attached. The resulting family of Superdex-based gels has been constructed to provide... 

Suitable affinity resins are CNBr-activated Sepharose and similarly activated jV-hydroxysuccimmide ester-based gels with spacer arms. These react with free amino groups on the peptide. If the peptide contains many lysine residues, alternative coupling systems may be used, for example, carbodiimide-activated agaroses. In our experience, however, even peptides with internal lysines make good immunoadsorbents, and we routmely use CNBr-activated Sepharose. 

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