Soil polysaccharides, chromatography

No structural work has been done on the polysaccharide preparations. In the hydrolyzates, the sugar components have been estimated quantitatively by paper chromatography or other methods (see Table II). It would be premature to try to interpret the ratios found between individual sugars or classes of sugars, or to discern a pattern in the variations between different soils, because the preparations were heterogeneous and repre.sented only a small part of the total polysaccharides of the soil. 

The isolated monosaccharides and polysaccharides represent only a small part of the total carbohydrates of soil. The soil residue after extraction, and isolated fractions of soil organic matter (for example, humic substances), might contain sugars other than those which have been detected in the polysaccharides isolated. However, hydrolysis of soils and of humic substances isolated, followed by chromatography of the freed sugars, showed that this was not the case. ... 

The elucidation of a levan polysaccharide produced by a novel halophilic isolate from soil samples taken from Camalti Saltern area in Turkey, Halomonas smyrniensis was achieved thanks to the glycosyl analysis. In particular, the microbial EPS was hydrolyzed with trifluoroacetic acid (0.5M TFA at temperature of 120°C for 1.5h) and subsequently analyzed by HPAE-PAD (High-Performance Anion-Exchange Chromatography Pulsed Amperometric Detection), a high-performance anion-exchange... 

Zhe X, Wenwei X, Hua H, Lirui P, Xu X (2008) Direct chiral resolution and its application to the determination of the pesticide tetramethiin in soil by high-performance liquid chromatography using polysaccharide-type chiral statitmary phase. J Chromatogr Sci 46 783-786... 

---