Polysaccharides, Oligosaccharides, and Glycopeptides

The reducing-end residue of several oligosaccharides has been labelled by reduction with sodium borotritiide, and oligosaccharides have been coupled to proteins, to give pseudoglycoproteins, following oxidation to the aldonic acids.  

Hirano and W. Onashi, Agric. and Biol. Chem. (Japan), 1976,40,2501. 

A peptidophosphogalactomannan (from Penicillium charlesii) immobilized by reaction with agarose cyclic imidocarbonate has been used as the affinity matrix in the purification of (exo-)p-D-galactofuranosidase.  

Oligosaccharide fragments have been obtained from pneumococcal Sill polysaccharide by mild acid hydrolysis.Pneumococcal Sill polysaccharide reacted with azo-(bovine serum albumin) to give a conjugate which, following immobilization on 0-bromoacetylcellulose, was used as an immunoadsorbent in the purification of anti-SIII antibodies.  

Miscellaneous Polysaccharides, Oligosaccharides, and Glycopeptides. — Lamin-aribiose has been modified to form the 3-methyl and (3-benzyI glycosides,and 4-0-o -D-mannopyranosyl-D-mannose has been chemically modified also.  

The blood group I and i activities of five synthetic oligosaccharides [(67)-(71)] have been investigated by radioimmunoassay.  

Sucrose has been activated by reaction with cyanuric chloride to yield a [ dsucrose-dichlorotriazine adduct which was stable for at least six hours and which could be covalently linked to low density lipoprotein.A general method utilizing radiolabelled sucrose covalently linked to protein is described for assessment of the degradation of metabolized proteins. 

Malto-oligosaccharides radioactively labelled at their reducing ends have been used to elucidate the mechanisms by which Aspergillus oryzae a-amylase degrades malto-oligosaccharides.  

The reducing ends of oligosaccharides have been coupled to 2-aminopyridine, a fluorescent compound, by reductive amination with sodium cyanoboro-hydride. The fluorescent oligosaccharides are then easily visualized after 

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