Polysaccharide lyases oligosaccharides

Oxidoreductases Transferases Hydrolases Lyases Isomerases Ligases Phenolic polymers, polyanilines, vinyl polymers Polysaccharides, cyclic oligosaccharides, polyesters Polysaccharides, polyesters, polycarbonates, poly(amino acid)s, polyphosphates... 

Polysaccharide lyases have been used to produce A -uronate containing disaccharides (Figure 4, la-lr) and higher oligosaccharides from heparin, heparan sulfate, chondroitin sulfates, dermatan sulfate, hyaluronan, and chemically modified GAGs. ... 

Polysaccharide lyases can be combined with separation methods such as chromatography and electrophoresis for the preparation of glycosaminoglycan oligosaccharides for biological evaluations as well as for disaccharide analysis, oligosaccharide mapping and polysaccharide sequencing. 

Figure 4. Depolymerization of heparin using polysaccharide lyase. The glycosidic linkage between a substituted glucosamine (X = H or SOa , Y = SO3 or CH3CO) and uronic acid (iduronic or glucuronic, X = SOa" or H) is cleaved eliminatively affording oligosaccharide products. The non-reducing end of each product formed contains a a-4,5 unsaturated uronic acid residue.

This enzyme [EC 4.2.2.3], also referred to as poly(j8-D-mannuronate) lyase and poly(mana) alginate lyase, catalyzes the eliminative cleavage of polysaccharides containing /3-D-mannuronate residues to give oligosaccharides with 4-deoxy-o -L-eryt/iro-hex-4-enopyranuro-nosyl groups at their ends. 

Alginate lyase from Turbo cornutus acted on sodium alginate and resulted in a rapid decrease in viscosity and a gradual increase in reducing power of the substrate solution. The result of gel filtration of the reaction products indicated that uronic acid oligosaccharides were liberated as the final product. The fact that the enzyme attacked preferentially the D-mannuronate-rich moieties of the alginate molecule was demonstrated by an analysis with paper electrophoresis of the reaction products after acid treatment, which separated the uronic acid oligosaccharides from the uronic acid polysaccharides. 

Lahaye, M., Bmnel, M., and Bormin, E. (1997) Fine chemical structure analysis of oligosaccharides produced by an ulvan-lyase degradation of the water-soluble cell-wall polysaccharides from Ulva sp. (Ulvales. Chlorophyta). Carbohydr. Res., 304,325-333. 

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