Polypeptides complete sequence analysis

Table 5.8 Polypeptides detected during the LC-electrospray-MS analysis of the tryptic digest from /Mactoglobulin ifiLG). Reprinted from J. Chromatogr., A, 763, Turula, V. E., Bishop, R. T., Ricker, R. D. and de Haseth, J. A., Complete structure elucidation of a globular protein by particle beam liquid chromatography-Fourier transform infrared spectrometry and electrospray liquid chromatography-mass spectrometry - Sequence and conformation of /Mactoglobulin , 91-103, Copyright (1997), with permission from Elsevier Science...

Amino acid analysis (196) and a complete amino acid sequence of the 33-kDa protein from spinach (201) indicated that no histidine was present. Thus, Mn ligation to this polypeptide must be to tyrosine phenoxide and/or to the carboxylate functionalities of aspartate and glutamate. 

With regard to racial variation, nothing has been definitely established. Hawk s data [22] appears to show subtle differences in the relative percentages of various amino acids found in the hydrolysates of Ethiopian hair as compared to Caucasian hair. Wolfram has compiled a more complete set of data from the literature of whole-fiber amino acid analysis of the three races, showing overlap in the amounts of all the amino acids from scalp hair for the three major racial groups [31]. Polypeptide isolation and amino acid sequencing rather than whole-fiber amino acid analysis will provide the best means for determining with certainty if any differences exist in the proteins of hair of different races. 

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