Polymerase chain reaction quality assurance

However, be aware that in spite of the usefulness of the MIB-1 antibody in assessing the rate of cell proliferation, the classification of cancers (e.g., breast cancer) by the size of the primary tumor and the presence and extent of lymph node metastases does not adequately explain differences in the clinical outcome of individual patients. Cell proliferation indices are commonly used, along with other diagnostic parameters, to estimate the risk of recurrence of a cancer for individual patients. Therefore, it is important to understand the relationship between various indices of proliferation such as MIB-1 labeling index and detection by either in situ hybridization or polymerase chain reaction. This approach will lead to quality assurance in diagnosis. 

Meyer R, Chardonnens E, HiibnerP, LiithyJ (1996). Polymerase chain reaction (PCR) in the quality and safety assurance of food detection of soya in processed meat products. Z. Lebensm. Enters. Eorsch., 203(4) 339-344. 

Kl. Keilholz, U., Willhauck, M., Rimoldi, D., Brasseur, F., Dummer, W., Rass, K., et al. Reliability of reverse transcription-polymerase chain reaction (RT-PCR)-based assays for the detection of circulating tumour cells A quality-assurance initiative of the EORTC Melanoma Cooperative Group. Eur. J. Cancer 34, 750-753 (1998). 

Immunoassays. Polymerase Chain Reaction. Proteins Overview. Quaiity Assurance Quality Control. 

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