Polymerase chain reaction , insect

It should be possible to develop either biochemical- or molecular-based tests for resistance in flatworms, as has been successfully used to detect insecticide resistance in insects. However, the necessary information is not available on the mechanism of resistance to be able to design tests of this type for use with trematodes or cestodes. When developed they will hopefully be based on real-time polymerase chain reaction (PCR) or pyrosequencing. 

L. dispar eggs. Small PTTH-specific nucleotide sequences have been amplified from L. dispar cDNA by the polymerase chain reaction (PCR), and fragments of the expect size have been obtained. The development of bioassays for L. dispar PTTH and recent work on PTTH in other insect species are discussed. 

Simon C, Paabo S, Kocher T, Wilson AC (1990) Evolution of the mitochondrial ribosomal RNA in insects as shown by the polymerase chain reaction. In Clegg M, O Brien S (eds). Molecular Evolution. UCLA... 

Simon, C., S. Paabo, T.D, Kocher, and A.C. Wilson. 1990. Evolution of Mitochondrial Ribosomal RNA in Insects as Shown by the Polymerase Chain Reaction. In M. Clegg and S. O Brien (eds.). Molecular Evolution, UCLA Symposia on Molecular and Cellular Biology, New Series, 1222 235-244. Wiley-Liss, New York. 

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