Polymerase chain reaction hybridization after

The dot-blot shown below examines DNA from a child with ambiguous genitalia after polymerase chain reaction (PCR) amplification and hybridization with DNA probes from the X and Y chromosome. In this case, the Y chromosome probe is from the SRY region of Yp that has recently been characterized as the male-determining region. DNA from control male and female patients is also applied to the dot-blot. Based on the dot-blot results, which is the most likely conclusion ... 

Detection of EHEC-associated markers is performed either directly from the whole genome (DNA-hybridization) or indirectly after amplification by Polymerase Chain Reaction (PCR) of specific gene fragments. 

To this end, cDNA libraries, prepared from cells or tissues known to be rich in certain receptors, were screened by low stringency hybridization, or were used for polymerase chain reaction (PCR) amplification of candidate genes using degenerate primers. Proof of function was obtained after the... 

A dipstick probe can be used to identify organisms in clinical or environmental specimens. It consists of adding a capture probe (containing a sequence of poly-dA or -dT bases that identifies it as a capture probe) coupled to dioxygenin as a reporter. Following destruction of unhybridized probe material, a dipstick that can hybridize with the capture probe base sequence is added. After all steps are completed, the presence of the capture probe is positive for the organism of interest. The theoretical sensitivity of a probe is < 1 pg of nucleic acid or 10 cells. However, by use of polymerase chain reaction (PCR), the DNA of a single cell can be amplified several millionfold. 

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