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Polymerase chain reaction -generated probes

The constitutive isoforms NOS I and NOS III are expressed by certain types of cells. When stimulated with cytokines (and some other agents), most cells seem to be capable of expressing the inducible NOS II. We have generated isoform-specific antibodies, oligonucleotides used as primers in reverse transcriptase-polymerase chain reaction (RT-PCR), and cDNA probes for all three isoforms to study the cellular and tissue distributions of the different isoforms. [Pg.173]

FIGURE 11.27 Scheme for capture, ligation, and ladder generation by polymerase chain reaction (PCR) on a duplex probe attached to a streptavidin-coated surface. [Pg.305]

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See also in sourсe #XX -- [ Pg.1432 ]



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Chains generation

Generation reactions

PROBE REACTION

Polymerase chain reaction -generated

Reaction polymerase

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