Marker Proteins for the Polyacrylamide Gel Electrophoresis

Prerequisites for the use of a protein or polypeptide as calibrator in PAGE are the accessibility in a pure, definite state, its known 

Since the obtained optical density (intensity of stain) per mass unit of a protein differs depending on its nature and the staining method, for some commercial available protein mixtures the composition is given, which produces staining a homogeneous picture by Coomassie. 

About 1 - 5 pi of the pre-mixed ready-to-use protein standards per lane are recommended in SDS-PAGE combined with Coomassie staining. 

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