Poly transfection efficiency

Peng SF, Yang MJ, Su CJ et al (2009) Effects of incorporation of poly(y-glutamic acid) in chitosan/DNA complex nanoparticles on cellular uptake and transfection efficiency. Biomaterials 30 1797-1808... 

Aral, C., Akbuga, J. (2003). Preparation and in vitro transfection efficiency of chitosan microspheres, containing plasmid DNA poly (L-lysine) complexes. J. Pharm. Phantn. Sci., 6(3), 321-326. 

Chemg, J.Y., Van de Wetering, R, Talsma, H., Crommelin, D.J.A., Hennink, W.E. (1996). Effect of size and serum proteins on transfection efficiency of poly ((2-dime-thylamino)ethyl methacrylate)-plasmid nanoparticles. Pharm. Res., 13, 1038-1042. 

Cherng, J.Y., Schuurmans-Nieuwenbroek, N.M., Jiskoot, W., Talsma, H., et al. (1999). Effect of DNA topology on the transfection efficiency of poly((2-dimethy-lamino)ethyl methacrylate)-plasmid complexes. J. Control Release, 60, 343-353. 

Polyfection was carried out with pCMVLuc (a 5 kb pDNA) on HepG2 cells for four hours at 37 °C. After 48 hours, the transfection efficiency was determined from Luciferase activity in cells measured by luminescence (RLU) and the cytotoxicity was evaluated by using the colorimetric MTT assay, dp is the poly lysine degree of polymerization. The transfection efficiency is scored on a 0 to 100 scale where 0 and 1 00 correspond to an absence of transfection and the most effective transfection, respectively compared to the pDNA alone. His is histidyl residue. 

Instead of poly lysine, commercially available oligolysine of dp 19, which is less polydisperse than poly lysine, has been used to condense pDNA. A minimal repeating lysine chain of 18 residues followed by a tryptophan and an alkylated cysteine (AlkCWK18) has been found to condense plasmid DNA into small particles of 78 nm that mediated efficient in vitro transfection (Wadhwa et al., 1997) (Table 16.4). Compared to a commercially available K19, AlkCWK18 induced a 40-fold reduction in particle size and a 1000-fold increase in transfection efficiency. 

Godbey, W.T., Wu, K.K., Hirasaki, G.J. and Mikos, A.G. (1999b) Improved packing of poly(ethylenimine)/DNA complexes increases transfection efficiency. Gene Ther., 6, 1380-1388. 

Various modifications to the poly(L-lysine) dendrimer have been investigated to improve transfection efficiency, such as the replacement of terminal lysine residues with either arginine or histidine or modification of the core [211, 212],... 

Keywords Applications Cytotoxicity profile Gene delivery Hyperbranched polyamines Poly(amido amine) Poly(amido ester) Poly(ethylene imine) Polyglycerol amines Structure-property dependence Synthesis Transfection efficiency... 

Furthermore, we studied the influence of the molecular weight and degree of branching on transfection efficiency and cell toxicity, which is supposed to have influence, as initially analyzed in several adherent cell lines (NIH/3T3, CHO-K1, COS-7, and HeLa), with unfunctionalized poly(ethylene imine)s [91, 95, 96]... 

Fig. 12 Transfection efficiency of the complexes of protonated poly(TMPTAl-AEPZ2)/DNA (pCMV-Luc) complexes in COS-7 and HEK 293 cells compared to that of PEI (25 kDa). The transfection efficiency of PEI (25 K) was obtained under an optimal N/P ratio of 10 1. Values were presented as mean standard deviation (n = 4). Reproduced with permission from [127]. Copyright 2005 ACS...

Kim TH, Kim SI, Akaike T et al (2005) Synergistic effect of poly(cthylcniminc) on the transfection efficiency of galactosylated chitosan/DNA complexes. J Control Release 105(3) 354-366... 

Kim TI, Seo HJ, Choi JS et al (2005) Synthesis of biodegradable cross-linked poly(beta-amino ester) for gene delivery and its modification, inducing enhanced transfection efficiency and stepwise degradation. Bioconjug Chem 16 1140-1148... 

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