Poly-L-lysine hydrobromide

Actual transfer distance along the primary DNA ds Interduplex center-to-center separation Spermine tetrahydrochloride Dodecyltrimethylammonium bromide Octadecyltrimethylammonium bromide Poly-L-lysine hydrobromide Polyethylenimine hydrochloride Ionization energy... 

Poly-L-lysine coated glass slides or coverslips. Clean slides or coverslips are dipped into a poly-L-lysine hydrobromide solution (0.5 mg/ml Sigma cat. no. P 1399), air-dried and stored in a dust-free environment at 4°C (at most for a week). 

Kikuchi K, Yoshioka K. Electric birefringence of poly-L-lysine hydrobromide in methanol-water mixtures and helix-coil transition induced by high electric fields. Biopolymers 1973 12 2667-2679. 

Darke, A., and Finer, E. G. (1975). Biopolymers 14, 441. Nmr Studies of Mixtures of Poly-L-Lysine Hydrobromide with Water. 

Poly-L-lysine hydrobromide (Sigma). Prepare a 0.1 mg/mL stock solution in water. Store at -20°C. 

Table 1 shows the details of the reagent masses that were used to synthesize the different polymers according to a method described in previous publications [12], Briefly, poly(L-lysine) hydrobromide (Fluka, Switzerland) was dissolved at a concentration of 100 mM in SBB (sodium borate buffer solution, 50 mM) adjusted to pH 8.5. The solution was filter sterilized (0.22 =m pore-... 

The PLL- -PEG copolymers were synthesized according to a previously described method (1, 2). Briefly, poly(L-lysine) hydrobromide (PLL-HBr, MW 20 kDa Sigma, St, Louis. MO) was dissolved in a 50 mM sodium borate buffer solution, followed by filter sterilization of the solution (0.22 m pore-size filter). The N-hydroxysuccinimidyl ester of methoxypolyiethylene gly-col)propionic acid (SPA-PEG Shearwater Polymers. Inc., Huntsville, AL) was then added to the dissolved PLL-HBr. The reaction was allowed to proceed for 6 h at room temperature, after which the reaction mixture was dialyzed (SpectraPor, MW cutoff size... 

When a mixture of ethyl and octadecyl sulfates are mixed with poly(L-lysine) hydrobromide, the complex that is formed contains exclusively octadecyl sulfate anions. The selective binding of the long surfactant chains is attributed to the hydrophobic driving forces that are the determinant for micellization. Con-... 

The PLL-g-PEG was dissolved in 10 mM HEPES (4-(2-hydroxyethyl)piperazine-1 -ethane-sulfonic acid, adjusted to pH 7.4 with 1.0 M NaOH solution) at a concentration of 0.25 mg/ml and this solution was used for ail pin-on-disk experiments. For the cases where the comparison of the PLL-g-PEG with the PLL(20) alone was examined, PLL(20) solution was prepared by dissolving the poly(L-lysine) hydrobromide in 10 mM HEPES at a... 

Poly-L-lysine-coated coverslips (poly-L-lysine hydrobromide Sigma)... 

Poly-L-lysine Hydrobromide (PLL) high (at least 600,000) molecular weight (Sigma Chemicals). 

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