Poly fluorescein-modified

A representative example of indirect imaging with SWNTs labeled with fluorescent molecules was illuminated by Dai and coworkers.102 They solubilized as-produced SWNTs via sonication with fluorescein-modified poly (ethylene glycol) (Fluor-PEG, 114 PEG units). The absorption maximum of the nanotube-attached Fluor-PEG was red shifted 3 nm from that of free fluorescein, and the fluorescence intensity was quenched about 67% in phosphate buffer solution (pH 7.4). In the bioevaluation with BT474 breast cancer cells, substantial intracellular fluorescence was observed, suggesting cellular uptake of the Fluor-PEG-functionalized SWNTs.102... 

Fluorescein tetramethylrhodamine dextran (FRD) was used as a ratiometric probe, which provides a pH-independent signal (tetramethylrhodamine), to compensate for different organelle volumes, and a pH-dependent signal (fluorescein). The ratio of the pH-independent and pH-dependent fluorescence was expected to provide a quantitative measure of the pH. Acidic organelles (lysosomes and endosomes) were specifically labeled by FRD, because the cells endocytose small amounts of the extracellular medium, which is accumulated in endosomes and eventually lysosomes. CE was performed in a poly((V-acryloyl aminopropanol, AAP) modified capillary to reduce both adsorption at the capillary surface and the electroosmotic flow. LIF detection was performed off-column using a... 

EKC separations of aliphatic amines include electrolyte systems composed of several surfactants (SDS, cholate, Brij 35 ) modified by certain additives (urea, neutral CDs, organic solvents ), mixed CDs, and more unusual secondary phases [resorcarene-octacarboxylic acid, calixarene," poly(sodium 4-styrenesulfonate), PSSS ]. Derivatization is performed when UV (o-phthaldialdehyde, OPA, as derivatizing agents) or LIF detection [3-(2-furoyl)quinoline-2-carboxaldehyde, 5-(4,6-dichloro-s-triazin-2-ylamino)fluorescein (DTAF) as labeling agents] is designed. 

Poly(amino acid)-based nanoparticles with different surface PEGylation were prepared. a,b-Poly(A-2-hydroxyethyl)-D,L-aspartamide (PHEAS) and PEG-modified PHEA (PHEAS-PEG) were functionalized with a methacrylate group and then polymerized by UV irradiation in inverse microemulsion. The resulting nanoparticles had a size of around 250 nm in diameter by TEM. The fluorescein-loaded PHEA-based nanoparticles were prepared in the presence of fluorescein sodium salts, and examined for cellular uptake using macrophage cells. ... 

Recent advances in detecting renal function include the development of fluorescently labeled novel compounds (hydrophilic pyrazine dyes, carbostyril 124-DTPA-Eu, fluorescein-labeled poly-D-lysine) and their detection via in vivo fluorescence imaging or through transcutaneous detection (Dorshow et al., 1998 Rabito et al., 2005 Rajagopalan et al, 2011 Poreddy et al, 2012). Both the hydrophilic pyrazine dyes (see Fig. 34.1) and the modified DTPA are cleared exclusively by glomerular filiation, and... 

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