Plasticware, washing

Table 9 summarizes procedures of blood collection for aluminum determination. Steps to avoid contamination, the analytical technique, and the aluminum levels found in the serum of healthy individuals are listed. It can be seen that, as a general procedure, plasticware was used, all materials that entered in contact with the sample were acid-washed, usually by nitric acid, and the analyses were carried out inside clean rooms. 

You will be directed to use kitchen utensils in many of the demonstrations. Don t assume that this means that the chemicals are nontoxic. Use plasticware and paper plates whenever possible (you will be told when only glassware will do) and throw the paper and plastic away after use. Even inexpensive paper plates will work for many of the demonstrations if they have a thin plastic coating. If you have to use glass or ceramic utensils, wash them thoroughly and separately before any contemplated reuse with food, or, better, maintain a separate set dedicated to chemical demonstrations. You will probably do most of the demonstrations in the basement, kitchen, or bathroom, but keep the chemicals well separated from toothbrushes, foodstuffs, or counters that will come in contact with food or toothbrushes. You may cover your counters with newspaper for added protection. 

Wash all glassware with concentrated H2SO4 and plasticware with 3 M HC1. All reagents and water should be of highest purity. 

Wash all glassware with concentrated H2S04 and plasticware with 3 m HC1. Follow all directions given in Procedure 4.6.a for Reaction 1. 

All glassware and plasticware must be acid-washed with concentrated H2S04 and 3 M HC1, respectively, to remove trace metal ions. Use purest H20 available for solutions. 

All glassware and plasticware must be acid-washed with concentrated H2S04 and 3 M... 

Wash all glassware with concentrated H2SO4 and plasticware with 3 M HC1. 

Regardless of the analytical method used, care must be taken to eUminate or prevent contamination of the sample by Hg during preparation and analysis. All glassware and plasticware used for collection and analysis of the specimen must be acid washed. In addition, care must... 

Poor washing applies to washing tips and glassware and plasticware. It is better not to reuse anything that has had contact with enzyme. When tips are reused, they must be acid washed and then very thoroughly rinsed in distilled water. The pH of the water should be checked because it can often be very... 

All plasticware used in sample preparation had been washed with a nonphosphate detergent, rinsed several times with deionized water, soaked overnight with 10% by volume nitric acid, rinsed several times with deionized watei, soaked overnight with deionized water, and rinsed several times with deionized water before use. All samples were stored at room temperature. Subsamples distributed to participants were obtained by shaking the sample container and pouring out 200 mL of suspension into washed plastic bottles. 

Precautions should be taken to ensure that all solutions, spatulas, plasticware, and glassware are sterile and free of any dust throughout the procedure. All materials must be RNase-free for all stages between the initial fixation and the post-hybridization washes (see Note 1). 

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