Rabbit plasma

Madu, A. Cioffe, C. Mian, U. Burroughs, M. Ibomanen, E. Mayers, M. Schwartz, E. Miller, M. Pharmacokinetics of fluconazole in cerebrospinal fluid and serum of rabbits Validation of an animal model used to measure drug concentrations in cerebrospinal fluid. AntimicrobAgents Chemother., 1994, 38, 2111-2115 [pharmacokinetics CSF serum plasma rabbit UK54373 (IS)]... 

A sensitive test method to detect nalmefene, an opioid antagonist in human and rabbit serum, using nalbuphine internal standard. The analyte is extracted using n-butyl chloride/acetonitrile (4 1). HPLC combined with electrospray and MS-MS detection is used for quantitative tests. First validated for human plasma, and cross-checked by rabbit serum and plasma. Specificities are 3.21, 5.55, and 3.62% for human plasma, rabbit plasma, and rabbit serum, respectively. Extraction yield 80% from human plasma. A calibration curve has been established for the 0.1-100 ng/ml concentration range. Within one run, at the lower limit the accuracy was 18% the precision was 13.6%. In the concentration range 0.3-75 ng/1 the accuracy was better than 128% the precision was 6.6% for aU matrix types. Intemm accuracy and precision 8.0 and 6.6%, respectively. The analyte remained stable after 24 h room temperature storage of three freeze-thaw cycles. 

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