Plasma-polymerized film, immobilized

Z.Y. Wu, Y.H. Yan, G.L. Shen, and R.Q. Yu, A novel approach of antibody immobilization based on n-butyl amine plasma-polymerized films for immunosensors. Anal. Chim. Acta 412, 29-35 (2000). 

K. Nakanishi, H. Muguruma, K. Ikebukuro, and I. Karube, A novel method of immobilizing antibodies on a quartz crystal microbalance using plasma-polymerized films for immunosensors. Anal. Chem. 68, 1695-1700 (1996). 

Preparation and Characterization of Active Glucose Oxidase Immobilized to a Plasma-Polymerized Film... 

This study on the immobilization of glucose oxidase and the characterization of its activity has demonstrated that a bioactive interface material may be prepared from derivatized plasma polymerized films. UV/Visible spectrophotometric analysis indicated that washed GOx-PPNVP/PEUU (2.4 cm2) had activity approximately equivalent to that of 13.4 nM GOx in 50 mM sodium acetate with a specific activity of 32.0 U/mg at pH 5.1 and room temperature. A sandwich-type thin-layer electrochemical cell was also used to qualitatively demonstrate the activity of 13.4 nM glucose oxidase under the same conditions. A quantitatively low specific activity value of 4.34 U/mg was obtained for the same enzyme solution by monitoring the hydrogen peroxide oxidation current using cyclic voltammetry. Incorporation of GOx-PPNVP/PEUU into the thin-layer allowed for the detection of immobilized enzyme activity in 0.2 M sodium phosphate (pH 5.2) at room temperature. 

Applications of thin plasma-polymerized films in microelectronics, gas separation, and catalysis, such as new types of immobilized polymer-based catalysts, have been already... 

Wang H., Liu Y., Yang Y., Deng T., Shen G., and Yn R., A protein A-based orientation-controlled immobilization strategy for antibodies nsing nanometer-sized gold particles and plasma-polymerized film. Anal. Biochem., 324(2), 219-226, 2004. 

The formation of hydrogel layers on conducting substrates via an electrochemical route can be achieved by free-radical polymerization initiated by an electron transfer from the substrate to a redox-active initiator. Such gels adhere well to the substrates. " Moreover, the use of plasma immobilization and electron-beam irradiation has been investigated for the preparation of stimuli-responsive hydrogel films. " " ... 

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