Plant cell culture chemical relationship

Plant Cells and Tissues Structure-Function Relationships. Methods for the Cytochemical/Histochemical Localization of Plant Cell/Tissue Chemicals. Methods in Light Microscope Radioautography. Some Fluorescence Microscopical Methods for Use with Algal, Fungal, and Plant Cells. Fluorescence Microscopy of Aniline Blue Stained Pistils. A Short Introduction to Immunocytochemistry and a Protocol for Immunovi-sualization of Proteins with Alkaline Phosphatase. The Fixation of Chemical Forms on Nitrocellulose Membranes. Dark-Field Microscopy and Its Application to Pollen Tube Culture. Computer-Assisted Microphotometry. Isolation and Characterization of... 

The new technique introduced new potential sources for studying the production of medicinally important compounds, their metabolic pathways and chemical regulation92. Activity in plant cell culture research has been considerably increased in the last decade 93). This technique is used in studies on problems in plant propagation and crop improvement. Host-parasite relationship studies are facilitated... 

Forty four alkaloids were newly isolated from the 22 lupin plants and the structures were elucidated in our recent study. The structures were determined by spectroscopic investigations, in particular, by extensive 2D-NMR experiments in the recent works, and by chemical transformation. Biosynthetic pathways are also proposed, in some cases, by the preparation of isolated enzymes and by in vitro cell culture system. Chemotaxonomic relationships of the lupin plants are discussed based on the structural classification of alkaloids. 

This process starts with the synthesis of novel chemical compounds. Substances with complex structures may be obtained from various sources, e.g., plants (cardiac glycosides), animal tissues (heparin), microbial cultures (penicillin G), or human cells (urokinase), or by means of gene technology (human insu-Un). As more insight is gained into structure-activity relationships, the search for new agents becomes more clearly focused. 

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