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Photoreactive crosslinkers interactions

In use, SAND is first reacted with an amine-containing protein or other molecule—being careful to protect the photoreactive group from inadvertent degradation by exposure to excessive room light or sun. The modified intermediate then is allowed to interact with a target molecule. Finally, the photolyzing process is done to effect a nonselective crosslink between the... [Pg.313]

SADP or sulfo-SADP also have been used to study the phenylalanine-methionine-arginine-phenylalanine-amide-activated sodium channel (Coscoy et al., 1998), various apolipoprotein E isoforms (Mann et al., 1995), the high-affinity phenylalkylamine Ca2+ antagonist binding protein from guinea pig (Moebius et al., 1994), the interaction of non-histone proteins with nucleosome core particles (Reeves and Nissen, 1993), and the interactions among cytochromes P-450 in the endoplasmic reticulum (Alston et al., 1991). See Chapter 28 for methods of using photoreactive heterobifunctional crosslinkers to study protein interactions. [Pg.316]

The number of commercially available crosslinkers for sulfhydryl and photoreactive conjugations provides enough variety to design successful experiments in photolabeling, such as studying active centers and macromolecular interactions. [Pg.325]

The homobifunctional photoreactive BASED (Chapter 4, Section 5.1) has two photoreactive phenyl azide groups, each of which contains an activating hydroxyl. Radioiodination of this crosslinker can yield one or two iodine atoms on each ring, creating an intensely radioactive compound. Crosslinks formed between two interacting molecules are reversible by disulfide reduction, thus allowing traceability of both components of the conjugate. [Pg.560]

The following sections discuss the application of several photoreactive heterobifunctional crosslinkers to the study of protein interactions. [Pg.1016]

Most recently, we reported small molecule arrays on photoaffinity crosslinker coated gold surfaces (17). The small molecule arrays were fabricated by photoreaction, and then analyzed by SPR imaging technique. The small molecules don t have to be modified chemically for immobilization. The small molecules, which can interact with a target protein, can be screened by this methodology. Therefore, the integration of photoaffinity small molecule array and SPR imaging technique can be the first step of reverse chemical genetics. [Pg.228]


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See also in sourсe #XX -- [ Pg.1016 ]




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