Phosphoproteomics

An important emerging strategy is the simultaneous utilization of SILAC-based approaches for the quantitative identification of legitimate PTMs. This approach has been used successfully in the interrogation of phosphorylation for the analysis of phosphoproteomic signaling.75 76... 

Mann M., Ong S.E., Gronborg M., Steen H., Jensen O.N., and Pandey A. (2002), Analysis of protein phosphorylation using mass spectrometry deciphering the phosphoproteome, Trend. Biotechnol. 20, 261-268. 

Frederick MJ, VanMeter AJ, Gadhikar MA et al (2011) Phosphoproteomic analysis of signaling pathways in head and neck squamous cell carcinoma patient samples. Am J Pathol 178(2) 548-571... 

Lu Y, Muller M, Smith D et al (2010) Kinome siRNA-phosphoproteomic screen identifies networks regulating AKT signaling. Oncogene 30 4567 577... 

Lavezzari G, Lackner MR (2012) Monitoring phosphoproteomic response to targeted kinase inhibitors using reverse-phase protein microarrays. Methods Mol Biol 795 203-215... 

Carr SA, Annan RS, Huddleston MJ. Mapping posttranslational modifications of proteins by MS-based selective detection Application to phosphoproteomics. In Burlingame AL, ed.. Mass Spectrometry Modified Proteins and Glycoconjugates, Vol. 405, New York Academic Press, 2005, 82-115. 

Human Proteinpedia provides a list of phosphopeptides identified in mass spectrometry-based phosphoproteomic studies. In addition, phosphorylation and dephosphorylation data curated from the literature are mapped to corresponding site and residue of sequences provided in HPRD. Using PhosphoMotif Finder one can analyze the presence of phosphorylation-based motifs, which are derived from the literature, in any protein of interest. This is a valuable data for biomedical investigators in the development of phospho-spedfic antibodies and peptide arrays. Availability of many raw MS datasets deposited in Human Proteinpedia... 

Label-free iTRAQ Global and targeted phosphoproteome analysis (190)... 

Sulfolobus solfataricus Thermophile Label-free Phosphoproteomics (219)... 

Morandell, S., Stasyk, T., Grosstessner-Hain, K., Roitinger, E., Mechtler, K., Bonn, G. K., and Huber, L. A. Phosphoproteomics strategies for the functional analysis of signal transduction. Proteomics 6, 4047 1056, 2006. 

Gruhlfr,A., Olsfn,).V., Mohammfd, S., Mortfnsfn, P., Fafrgfman, N. )., Mann, M., Jensen, O. N. (2005). Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway. Mol. Cell Proteomics 4, 310-327. 

Below Scaled-up sections l-V of the 2D gels. This figure clearly demonstrates the power of autoradiography in the differential phosphoproteome analysis. 

Identification of a New Therapeutic Target for Anti-Inflammatory Therapy by Analyzing Differences in the Phosphoproteome of Wild Type and Knock Out Mice... 

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