Phospholipids in serum

Quantitative measurement of phospholipids is rare in routine clinical practice but more common in research (e.g., in studies of dietary influences). The choline-containing phospholipids lecithin, lysolecithin, and sphingomyelin, which account for at least 95% of total phospholipids in serum, are readily measured by an enzymatic reaction sequence using phospholipase D, choline-oxidase, and horseradish peroxidase. Kit methods with this enzymatic sequence are available commercially. Before the availability of enzymatic reagents, the common quantitative method involved extraction and acid digestion with analysis of the total lipid-bound phosphorus. ... 

When enzyme sensors are combined with FIA they have applications in medicine and the food produce industry. For example, FIA has been combined with the glucose oxidase electrode to determine glucose in blood [270], with the choline oxidase electrode for the determination of cholinesterase in serum [271], and with a bienzyme electrode that uses choline oxidase and phospholipase for the determination of phospholipids in serum [272]. The technique has also been used with an alcohol oxidase electrode to determine alcohol in drinks (for example, beers) [273], or with a glucose oxidase electrode to determine glucose [274] in various foodstuffs [275] or in fermentation processes [276]. 

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See also in sourсe #XX -- [ ]

See also in sourсe #XX -- [ , , , , ]

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