Phosphate-buffered saline containing azide

The in vitro release kinetics of numerous growth factors and cytokines were initially determined in phosphate-buffered saline containing azide (PBSA, pH 7.4). Figure 7 depicts the release kinetics of recombinant human tumor necrosis factor-p (rhTNF-p), recombinant human transforming... 

Storage solution (phosphate-buffered saline [PBS], pH 7.2, with 0.02% sodium azide, each liter contains 8 g of NaCl, 0.2 g of KC1, 1.44 g of Na2HP04, and 0.24 g of KH2P04). (Caution sodium azide is very toxic read the label prior to use and handle with care.)... 

PBSN3, phosphate-buffered saline (PBS) containing v/v 0.1% sodium azide. 

Procedure. Mix the ligand and bead suspension at 4°, then add EDAC. Allow the mixture to rock at 4° for 4-25 hr. In the cold, wash the beads with three 20-ml portions of coupling buffer, three 20-ml portions of 5 M guanidine hydrochloride, pH 7.2, then several times with phosphate-buffered saline, pH 7.2. After the beads have stood in this last buffer at 4° for 2 hr, they are washed twice with 20 ml of VBS-gel and resuspended in 25 ml of this buffer containing 0.04% sodium azide and stored at 4°. Generally, beads are stable for several months and can be prepared with reproducible activity. 

The antimicrocystin-LR monoclonal antibody-combined gel was prepared as follows Affi-Gel 10 (25 mL, Bio-Rad, Hercules, CA, U.S.A.) was washed with distilled ice water (250 mL) and mixed with an equal volume of 10 mg/mL of M8H5 antimicrocystin-LR monoclonal antibody in phosphate buffer saline (PBS) (pH 7.4). The gel mixture was incubated at 4°C for 24 h with gentle rocking. After the addition of 1 M ethanolamine hydrochloride (2.5 mL), the gel mixture was washed with distilled water (250 mL), followed by PBS (500 mL). The obtained gel cake was suspended in PBS containing 0.1% sodium azide (50 mL) and stored at 4°C. The gel mixture... 

Flow cytometry wash buffer standard phosphate-buffered saline (PBS, pH 7.4) containing 0.2% sodium azide, 0.1 % bovine serum albumin, and 2% human AB+ serum. Filter by suction through a 0.45-pM filter. 

PBS (phosphate buffered saline), pH 7.40 for liposome preparation (for cases in which empty liposomes are prepared). This buffer is also used as elution buffer, for cleaning ARSL (from non-encapsulated molecules) by gel filtration. In each liter, this buffer contains sodium phosphate (Sigma) 0.05 M, NaCl 150 mM and sodium azide (Sigma) 0.2 g (to a final concentration of 0.02% w/v for prevention of bacterial growth). Before adjusting the volume (to 1 L), the pH ofthe solution is adjusted to 7.40. 

Assay Diluents As the noncovalent interactions between antibody and analyte are influenced by pH, ionic strength, and temperature, typical assay buffers are isotonic solutions at or near neutral pH. Assay buffer and diluent formulations should not only promote analyte antibody interactions but also minimize the nonspecific interactions between the critical reagents and the variety of biomolecules in the sample matrix. Components such as BSA, HSA, and nonionic detergents are often included in assay buffers. Phosphate buffered saline (PBS) or 10 mM Tris-HCl solutions (both near pH 7.4) containing 1% BSA and 0.05% Tween-20 are common buffers that can also be used for dilution of test samples and detection reagents, as well as wash buffers. When wash buffers are used in large quantities and stored at room temperature, preservatives such as sodium azide or thimerasol are often added to increase their shelf life. It should be noted, however, that some components of the wash buffer may have adverse effects... 

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