Phenotypic assays technology

Mutations in HIV accumulate and interact with each other and cause resistance to one drug and then others, one of the pivotal problems in treatment. In the past, the complexity of HIV drug-resistance testing and the limited information on its clinical utility made routine application impractical. Recent advances in automated assay technology have allowed rapid characterization of HIV in blood samples, so an increasing number of commercial laboratories now offer phenotypic and genotypic testing. 

Table 6.6 lists voltage-gated sodium channelopathies (disease, mutations and phenotype), Table 6.7 describes the VGSC gene family and Table 6.8 describes ion-chatmel assay technologies, such as electrophysiology, membrane-potential measurement, flux assays, and binding assays. 

Table 4.1 Common assay technologies and perturbation measured in a phenotypic assay [11].

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