Phage display perspectives

Pannekoek H, van Meijer M, Schleef RR, Loskutoff DJ, Barbas CD, Functional display of human plasminogen-activator inhibitor I (PAI-1) on phages Novel perspectives for structure-function analysis by error-prone DNA synthesis, Gene, 128 135-140, 1993. 

This technique represents an innovative method to isolate antibodies against novel membrane molecules and epitopes expressed in their native conformation and allows the identification of new tissue-specific targeting molecules. From this point of view, this approach opens a new perspective in phage display technology and represents the first key step toward in vivo application. 

As well as those methods adumbrated, which give either a structural or a thermodynamic perspective, there are methods which are more indirect, affording inferential insights rather than a direct and relatively unequivocal readout. These include both library-based and so-called genetic methods. Library methods are typified by perhaps the most widely known method in PPIs the so-called yeast 2 hybrid, or Y2H system, and the other well-known approach phage display and... 

In this section, we will briefly highlight the successes that we have achieved with chemokine phage display and the perspectives for further use of the analogs that were isolated. 

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