Petunia transformation

Meyer P, Heidmann 1, Forkmann G et al (1987) A new petunia flower colour generated by transformation of a mutant with a maize gene. Nature 330 677-678... 

Not all attempts at metabolic engineering deliver the expected results. For example, Liicker et al. [11] transformed petunia (Petunia hybrida) with the (S)-linalool synthase (LIS) gene from Clarkia breweri (Scheme 26.1), but despite... 

The entire tissue culture sequence, used to obtain the transformed petunia plants, Is shown in Figure 18. Two types of callus are growing in the petri plate containing kanamycin media. 

Figure 18. Tissue culture sequence to obtain transformed petunia plants expressing a foreign gene, kanamycin resistance. The petri plate at the bottom contains two calli. The callus not forming shoots received the "long transfer", and the shoot-forming callus, the "short transfer". The "short transfer" shoots are removed from the callus and rooted in the container in the center. The rooted plant is transferred to the greenhouse. The leaves of the regenerated plant express the foreign gene.

Figure 9.154 Detection of CAT activity in transformed rice and petunia protoplasts by HPLC. (a) Petunia leaf, (b) Petunia cell suspension, (c) rice leaf, and (d) rice cell suspension protoplasts electroporated at 500, 625, 825, and 625 V with an 860 fiF capacitor, respectively. Protoplasts were transformed by electroporation with 20 figl mL pDW2. Protein extracts were prepared and used in a 200 /xL CAT reaction mixture. Typically 12 fig of crude protein was used per reaction except for rice cell suspension protoplasts, where 20 fig was used. Products were isolated and 1 to 5 fiL of purified products was injected on top of the column. Peaks C, chloramphenicol E, solvent peak, ethyl acetate M, 1- and 3-monoacetoxy chloramphenicol. (From Davis et al., 1992.)...

The transformed tobacco cells react in a very sensitive and specific way on brassinosteroid-treatment. Various non-transformed, hormone-heterotrophic callus cultures such as wild-type tobacco (strain SR7), carrot and Petunia showed rather undefined or weak reaction upon the application of BR (7). 

Fraley, R.R., R.B. Horsch, A. Matzke, M-D Chilton, W.S. Chi 1 ton, and P.R. Sanders. 1984. In vitro transformation of petunia cells by an improved method oT co-cult1 vation with A. tumefaciens strains. Plant Mol, Biol. 3 371. 

---