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Clarkia breweri

The Arabidopsis GES protein shares 40% sequence identity with two characterized linalool synthases from Clarkia breweri and Clarkia concinna [65], which together belong to the TPS-f family (Fig. 11.4). Linalool synthases catalyze a reaction analogous to that of GES by converting the 10-carbon substrate GPP into... [Pg.167]

Figure 10.1. The gene from Clarkia breweri coding for (S)-linalool synthase (LIS) was added to three different piant species (tomato, petunia and carnation) and each species produced the expected product, S-iinaiooi. However, the existing NP metabolic flexibility in each species aiiowed the novei substance, S-iinaiooi, to be converted to other substances, those substances being different in each species due to the differences in NP metabolism in each plant. ... Figure 10.1. The gene from Clarkia breweri coding for (S)-linalool synthase (LIS) was added to three different piant species (tomato, petunia and carnation) and each species produced the expected product, S-iinaiooi. However, the existing NP metabolic flexibility in each species aiiowed the novei substance, S-iinaiooi, to be converted to other substances, those substances being different in each species due to the differences in NP metabolism in each plant. ...
Not all attempts at metabolic engineering deliver the expected results. For example, Liicker et al. [11] transformed petunia (Petunia hybrida) with the (S)-linalool synthase (LIS) gene from Clarkia breweri (Scheme 26.1), but despite... [Pg.617]

Raguso, R. A., Light, D.M. and Pichersky, E. (1996). Electroantennogram responses of Hyles lineata (Sphingidae Lepidoptera) to floral volatile compounds from Clarkia breweri (Onagraceae) and other moth-pollinated flowers. Journal of Chemical Ecology 22 1735-1766. [Pg.175]

Raguso R. and Pichersky E. (1995). Floral volatiles of Clarkia breweri and C. concinna (Onagraceae) recent evolution of floral aroma and moth pollination. Plant Systematics and Evolution 194, 55-67. [Pg.16]

Originally isolated and characterized from the petals of Clarkia breweri, salicylic acid carboxyl methyltransferase (SAMT), converts salicylic acid to methylsalicylate.14 Methysalicylate, a volatile ester, has a characteristic wintergreen scent Apart from its putative role in defense, methylsalicylate is an important chemoattractant for moth-pollinated flowers and one of the main volatiles released from Clarkia breweri flowers.44,55 SAMT is a member of the type 3 family of plant MTs. Similarly to the previously discussed families of methyltransferases, type 3 MTs are homodimeric proteins. [Pg.52]

DUDAREVA, N., RAGUSO, R.A., WANG, J., ROSS, J.R., PICHERSKY, E., Floral scent production in Clarkia breweri. III. Enzymatic synthesis and emission of benzenoid esters, Plant Physiol., 1998,116, 599-604. [Pg.58]

DUDAREVA, N., CSEKE L., BLANC, V.M., PICHERSKY, E., Evolution of floral scent in Clarkiar. Novel Patterns of S-linalool synthase gene expression in Clarkia breweri flowers., Plant Cell, 1996,8,1837-1848. [Pg.199]

D AURIA, J. C., CHEN, F., PICHERSKY, E., Characterization of an acyltransferase capable of synthesizing benzylbenzoate and other volatile esters in flowers and damaged leaves of Clarkia breweri., Plant Physiol., 2002,130,466-476. [Pg.283]

Dudareva, N., D Auria, J.C., Nam, K.H., Raguso, R.A. and Pichersky, E. (1998) Acetyl-CoA benzylalcohol acetyltransferase an enzyme involved in floral scent production in Clarkia breweri. Plant., 14, 297-304. [Pg.234]

Blanc, V.M. and Pichersky, E. (1995) Nucleotide sequence of a Clarkia breweri cDNA clone of ipil, a gene encoding isopentenyl pyrophosphate isomerase. Plant Physiol, 108, 855-6. [Pg.288]

THOLL, D., KISH, C.M., ORLOVA, 1., SHERMAN, D., GERSHENZON, J., PICHERSKY, E., DUDAREVA, N., Formation of monoterpenes in Antirrhinum majus and Clarkia breweri flowers involves heterodimeric geranyl diphosphate synthases., Plant Cell, 2004,16, 977-992. [Pg.25]

Pichersky, E., Lewinsohn, E., and Croteau, R., Purification and characterization of S-linalool synthase, an enzyme involved in the production of floral scent in Clarkia breweri,Arch Biochem. Biophys., 316, 803, 1995. [Pg.309]

Volatile compounds have been found to be synthesized de novo in the cells of the plant tissues from which they are emitted [131, 132]. In flowers, their biosynthesis occurs in the epidermal cell layers, allowing easy escape of volatiles into the atmosphere [133-135]. While in some species, including Clarkia breweri and some rose varieties (e.g. Rosa x hybrida Baronne Edmond de Rothshild , Anna , The Mac Cartney Rose ), the biosynthesis of volatile constitnents of scent is localized on either side of the petals [136, 137], in others (snapdragon, Stephanotis floribunda, Nicotiana sua-veolens and Rosa x hybrida Lady Hillingdon ),... [Pg.417]

Fig. 6 Different species-specific fates of linalool produced via ectopic expression of Clarkia breweri lina-lool synthase (LIS) [132] in tomato [200], petunia [187] and carnation [188]. GPP, geranyl diphosphate... Fig. 6 Different species-specific fates of linalool produced via ectopic expression of Clarkia breweri lina-lool synthase (LIS) [132] in tomato [200], petunia [187] and carnation [188]. GPP, geranyl diphosphate...

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