Tetra peptide

Fig. 7. High energy ms/ms product ion spectmm of a hexylated tetra-peptide (33,34).

On this basis (see Fig. 2.41), various y9-di- and tetra-peptides incorporating [f-HLys-yS -HTrp (e.g. 160 [99], 161 [254]) or y9 -HTrp-y9 -HTrp, a-Trp-y9 -Lys [254] sequences as well as y -dipeptides with a Trp side-chain in the y -position for the first residue and a Lys side chain in the y" -position for the second residue (e.g. 

Tropoelastin is the soluble precursor of elastin and consists of alternating hydrophobic and hydrophilic peptide domains. The most common amino acids in the hydrophobic domains are Gly, Val, Ala, and Pro, which are often present in repeats of tetra-, penta-, and hexapeptides, such as Gly-Gly-Val-Pro, Gly-Val-Gly-Val-Pro, Gly-Val-Pro-Gly-Val, and Gly-Val-Gly-Val-Ala-Pro, respectively [3, 4]. The hydrophilic domains are mainly composed of lysines interspersed by alanines. 

To get site-specific information about this unfolding process, a similar series of peptides, Ac-(AAAAK)3AAAA-YNH2, was sequentially labeled with 13C on the C=0 of four Ala residues, first on those at the N terminus, and then sequentially for the three other tetra-Ala positions (Decatur and Antonie, 1999). IR results showed a band shifted down 40 cm-1 from the amide F, which formed a clearly resolved band... 

In the absence of further targeting information, the expressed protein is secreted to the apoplast. The stability of antibodies in the apoplast is lower than in the lumen of the ER. Therefore, antibody expression levels can be increased up to ten times higher if the protein is retrieved to the ER lumen using an H/KDEL C-terminal tetra-peptide tag [133]. Again, although the principles of ER-retention in molecular farming have been established using antibodies, it is likely that they will also apply to many other proteins. 

In order to obtain the biologically interesting sialyl TN antigen glyco-peptides, 7V-(benzyloxycarbonyl)-C -[methyl(5-acetamido-4s7,8,9-tetra-C -acetyl-3,5-dideoxy-D-g/ycmM -D-gtf/0cfo-2-nonulopyranosyl)-onate-(2 — ... 

It is too early to draw any conclusions about the insensitivity of the rate constants to the nature of the dipeptide. Differences among the peptides seem to be revealed more in the temperature dependencies of the rate constants for intramolecular electron transfer than in the magnitude of the rate constant itself. Work is in progress on the synthesis of other di-, tri-, and tetra-peptides separating Co(III) and Ru(II) in order to examine the temperature dependence of the intramolecular rate... 

Ni" forms square-planar bis-complexes with the amidate anions of L-Val, L-Phe, and L-Pro. The structure of bis(Gly)-bis(imidazole)nickel(ii) has been reported and the configuration around the metal atom is cis-O(carboxyl), cis-N(amine), cis-N(imidazole). Tetra- and penta-peptide complexes of nickel(ii) consume oxygen in neutral solutions as the metal ion catalyses peptide oxidation to give a number of products, including amides of amino-acids and peptides, oxo-acids, and C02- ... 

Reduction of a-Glycosyl Azide and Peptide Coupling /V -Fmoc-Asn[tetra-0-Bzl-a-D-Glc-(1 6)-tri-0-Piv- J-D-Glc-(l—>6)-tri-0-Bzl-a-D-Glc-(l—d-AJ-Pro-OtBu 4081... 

The coding sequences of a- and P-globins of human hemoglobin have been fused to the sequence of the chloroplastic transit peptide of the small subunit of Rubisco. These proteins were then coexpressed in transgenic tobacco plants, resulting in the production of a functional form of tetra-meric hemoglobin. The results demonstrate that a complex multimeric protein such as recombinant human hemoglobin can be obtained from tobacco in a functional form. 

Additional support for the single receptor model (Figure 12) comes from experiments that utilized various peptide mixtures. For example, when the N-terminal di- tri- or tetra- peptide are removed from BMP the remaining peptide... 

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