Peptides termination

Further, this method allowed an asymmetric construction of non-coded a,a-dialkyl-a-amino acid residues at the peptide terminal, as exemplified by the stereoselective alkylation of the dipeptide, L-Ala-L-Phe derivative 59 (Scheme 5.29) [31]. 

Sequencing the Peptide Terminal Residue Analysis The amino acid analyzer determines the amino acids present in a peptide, but it does not reveal their sequence the order in which they are linked together. The peptide sequence is destroyed in the hydrolysis step. To determine the amino acid sequence, we must cleave just one amino acid from the chain and leave the rest of the chain intact. The cleaved amino acid can be separated and identified, and the process can be repeated on the rest of the chain. The amino acid may be cleaved from either end of the peptide (either the N terminus or the C terminus), and we will consider one method used for each end. This general method for peptide sequencing is called terminal residue analysis. 

Peptides, Terminal and Sequence Studies in. Recent Developments in Techniques for... 

Determination of structure of peptides. Terminal residue analysis. 

Nucleophilic addition of the peptide terminal amino group to phenyl isothiocyanate (PITC) yields an N-phenylthiourca derivative. 

GalNAc4TAP24 represents the TAP24 peptide terminally glycosylated with GalNAc-T2, and GalNAc attachment site are underlined [49]. 

---