Peptides, metal arrays

Supramolecular Metal Arrays on Artificial Metallo-DNAs and Peptides... 

Recently, we have demonstrated that biopolymers such as DNA and peptides can be chemically reconstructed, and thereby act as template molecules for homogeneous or heterogeneous metal arrays in a programmable manner. This chapter covers our recent approaches to metal arrays on artificial DNAs and cyclic peptides. 

SUPRAMOLECULAR METAL ARRAYS ON ARTIFICIAL METALLO-DNAs AND PEPTIDES... 

Keywords Amino add Peptide Self-assembly Metal array Supramolecular gel... 

The SPOT-synthesis method also employs Fmoc chemistry but uses hydroxyl groups present on cellulose filter paper to derivatize and thereby immobilize (3-alanine groups onto the paper. After deprotection, the 13-alanine groups can be used as platforms for the synthesis of peptide arrays (Fig. 7.5) (Frank, 1992 Gausepohl et al., 1992). This method has been widely used for mapping antigen-antibody interactions as well as protein-DNA, protein-metal and other protein-protein interactions (Reineke et al., 2001). 

Fu X, Wang Y, Huang L, Sha Y, Gui L, Lai L, Tang Y. Assemblies of metal nanoparticles and self-assembled peptide fibrils—formation of double helical and single-chain arrays of metal nanoparticles. Adv Mater 2003 15 902-906. 

Peptide-modified electrodes for other metals towards multianalyte arrays... 

The ability to detect Cu2+, Cd2+ and Pb2+ with different electrodes modified in the same way but with different peptides selective for different metals opens the door to fabricating an electrode array for more than one metal ion. To explore that possibility an electrode array with four elements was employed where each electrode was modified with thioctic acid. The four elements were electrodes modified with (a) no peptide (that is thioctic acid alone), (b) Gly-Gly-His, (c) GSH and (d) human angiotensin I (see Fig. 10.5). The electrode array was calibrated by preparing 16 different cocktails of Cu2+, Cd2+ and Pb2+ according to a central composite design [53]. OSWV for each electrode for one of the calibration solutions (0.100 pM Cu2+, 3.00 pM Cd2+ and 0.600 pM Pb2+ in 50 mM ammonium acetate at pH 7.0) are shown in Fig. 10.6. The two features apparent from Fig. 10.6 are that there are only two main peaks due to the overlap of the electrochemistry from... 

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