Synthetic peptide receptors

Hart BR, Shea KJ. Synthetic peptide receptors molecularly imprinted polymers for the recognition of peptides using peptide-metal interactions. J Am Chem Soc 2001 123 2072-2073. 

Figure 3. Synthetic scheme for the preparation of porphyrin-amino acid or porphyrin- peptide receptors 2-11 for targeting protein surfaces. 1 (TCPP) and other TPP variants are conveniently prepared using the Lindsey procedure (BF3, methylene chloride, dilute). The carboxylic acids provide an attachment site for various amino acids and peptides, via amide bond formation. X corresponds to appropriately protected amino acids to make 2-11.

Asperlicin is moderately potent, poorly soluble in water, and not bioavailable by the oral route (41). When discovered it was also, with morphine, one of the very few nonpeptides with affinity for a peptide receptor (peptoids are discounted in this assessment). It was an interesting target for synthetic modification, particularly viewed as a benzodiazepine derivative with potential CNS activity. 

Antibiotics, immune suppressive agents, antibodies for specific agonist receptors, synthetic peptide mimetics of interactive domains of cell matrix components, such as collagen, fibronectin fibrinogen, ticlopidine, disintegrins, proteins firom venoms, proteins from saliva of blood sucking animals and insects. 

Bioassays, receptor assays, and immunoassays are used to measure GH. ° Most bioassays assess GH activity by analyzing a variety of growth-related metabohc changes in hypophysectomized rats or in cell cultures. A relatively simple and convenient bioassay, the Nb2 cell assay, uses cultures of GH-sensitive rat lymphoma cells.These bioassays are used primarily for calibrating GH reference materials or for comparing the biological potency and immunoreactivity of GH fragments and synthetic peptides. Receptor assays have also been applied to the measurement of GH, but their use has been limited to research applications. 

Ramstrom, O. Nicholls, LA. Mosbach, K. Synthetic peptide receptor mimics—highly stereoselective recognition in noncovalent molecularly imprinted polymers. Tetrahedron Asymmetry 1994, 5 (4), 649-656. 

Synthetic peptides can be used to isolate receptors for many hormones and other signal molec ules. For example, white blood cells are attracted to bacteria by formylmethionyl (fMet) peptides released in the breakdown of bacterial proteins. Synthetic formylmethionyl peptides have been useful in identifying the cell-surface receptor for this class of peptide. Moreover, synthetic peptides can be attached to agarose beads to prepare affinity chromatography columns for the purification of receptor proteins that specifically recognize the peptides. 

SYNTHETIC PEPTIDE RECEPTORS NONCOVALENT INTERACTIONS INVOLVING PEPTIDES... 

SYNTHETIC PEPTIDE RECEPTORS NONCOVALENT INTERACTIONS INVOLVING PEPTIDES Hans-Jdrg Schneider, Frank Eblinger, and Mallena Sirish... 

There has been much recent interest in biomimetic systems, i.e. those that mimic the degree of selectivity observed in biological systems. The formation of a molecular imprint of either an agonist or an antagonist of a particular receptor gives rise to a site that can be considered akin to the receptor, or part of the receptor. Such imprints have been termed receptor mimics . Synthetic peptide receptor mimics [100] were highly stereoselective when used as HPLC stationary phases. Andersson et al. [101] used imprints of the en-... 

Ramstrom O, Nicholls I A, Mosbach K (1994) Synthetic peptide receptor mimics highly stereoselective recognition in nonconvalent molecularly imprinted polymers. Tetrahedron Assymetry 5(4) 649—656... 

Since linear chains of amino acids can assume many conformations at their receptors, it is difficult to define the sequences and their steric relationships that are critical for activity. Thus, development of nonpeptidic synthetic agonists or antagonists that interact with specific peptide receptors has been difficult similarly, morphine is only natural product that acts selectively at peptidergic synapses. 

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