Peptide immunization with Freund

Fig. 15 Induction of cellular immunity by subcutaneous immunization with OVA-encapsulating y-PGA-Phe nanoparticles. Mice were subcutaneously immunized one time with OVA alone (10 pg), 10 pg of OVA and 100 pg of NPs (OVA-NPs), 10 pg of OVA and 100 pL of complete Freund s adjuvant (OVA + CFA), or PBS (control). Splenocytes were obtained from the immunized mice on day 10 after the immunization and stimulated with the OVA peptide. The number of IFN-y-producing cells was measured by enzyme-linked immunospot assay. SFU spot forming units...

Serum antibodies were obtained by immunization with free peptide in complete Freund s adjuvant. 

We have studied the effect of several adjuvants on the antibody responses to several of the peptides (52,53). In general, the highest responses were obtained in immunization of peptide with complete Freund s adjuvant. Immunizations without adjuvant (in PBS) or with Incomplete Freund s adjuvant usually gave little or no responses. 

Thtee C57BL/6 mice pet gtoup wete injected inttadermally with 50 pg of liposomal plasmid DNA (A, C) ot 50 pg free plasmid DNA (B, D) followed by a second treatment after 48 h. Three control mice received 50 pg of an antiviral peptide antigen (gp33) in incomplete Freund s adjuvant (IFA) inttadermally. Nine days after the first immunization, spleen cells were re-stimulated for 5 days and analyzed in a Cr-release assay on peptide labeled and unlabeled EL-4 cells. The spontaneous Cr-release was <14% (22). 

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