Pasteurella multocida, enzyme from

HA synthase from Streptococcus equisimilis was employed for milligram-scale synthesis of HA (109). In this reaction system, UDP-sugars (UDP-GlcA and UDP-GlcNAc) were effectively regenerated by the catalyses of several enzymes, synthetic HA was produced in 90% yield. In addition, mutated HA synthase from Type A Pasteurella multocida (PmHAS 1-703 aa) was recently used for the stepwise synthesis of HA, which has a monodispersed molecular mass of up to 20 sugar units (110). 

A unique HAS enzyme occurs in the virus that infects an algae Pasteurella multocida (pmHAS) that is quite different from all other HA synthases [94]. This is in a class by itself and will not be considered further. 

Our group has reported a novel multifunctional bacterial enzyme, Pasteurella multocida sialyltransferase (PmSTl). PmSTl is an easy to express extremely soluble, and highly active sialyltransferase. Routinely, about 100 mg of PmSTl can be purified from one liter E. coli cell culture. PmSTl has four different activities including a2,3-sialyltransferase (optimal activity at pH 7.5-9.0), a2,6-sialyltransferase (optimal activity at pH 4.5-7.0), a2,3-sialidase (optimal activity at pH 5.0-5.5), and trans-sialidase (optimal activity at pH 5.5-6.5) activities. Among them, a2,3-sialyltransferase activity is the major function of the PmSTl with a specific activity of 60 U/mg protein (24),... 

We have utilized the enzymes derived from a certain bacterial species, Pasteurella multocida, with favorable properties to create biomaterials suitable for a variety of health applications. Our chemoenzymatic methodologies allow the preparation of either defined oligosaccharides or narrow size distribution polysaccharides with a variety of GAG compositions. 

Example of the one-pot, three-enzyme synthesis of Siaa2-6GalbpNP (32) and Siaa2-3GalppNP (33). Abbreviations Pm NeuA aldolase from, Pasteurella multocida NmCSS CMP-synthetase CMP-sialic acid synthetase from Neisseria meningitidis PmSTI P. multocida sialyltransferase Pd2,6ST a2-6-sialyltransferase from Photobacterium damsela GaippNP p-nitrophenyl p-galactoside. 

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