Paper, starch analysis

Paper/starch first analysis of nucleotides and chemical synthesis products inherited changes in proteins + antisera- antigen recognition (Ouchterlony method)... 

In the context of the stability of the nitrosoamine intermediate in the diazotization of heteroaromatic amines relative to that in the case of aromatic amines, the reversibility of diazotization has to be considered. To the best of our knowledge the reverse reaction of a diazotization of an aromatic amine has never been observed in acidic solutions. This fact is the basis of the well-known method for the quantitative analysis of aromatic amines by titration with a calibrated solution of sodium nitrite (see Sec. 3.3). With heteroaromatic amines, however, it has been reported several times that, when using amine and sodium nitrite in the stoichiometric ratio 1 1, after completion of the reaction nitrous acid can still be detected with Kl-starch paper,... 

In a paper that addresses both these topics, Gordon et al.11 explain how they followed a com mixture fermented by Fusarium moniliforme spores. They followed the concentrations of starch, lipids, and protein throughout the reaction. The amounts of Fusarium and even com were also measured. A multiple linear regression (MLR) method was satisfactory, with standard errors of prediction (SEP) for the constituents being 0.37% for starch, 4.57% for lipid, 4.62% for protein, 2.38% for Fusarium, and 0.16% for com. It may be inferred from the data that PLS or PCA (principal components analysis) may have given more accurate results. 

W.A.Schroeder et al, IEC 43, 941-3(1951) 8) W.C.Sumpter F.M.Miller, Heterocyclic Compounds with Indole and Carbazole Systems , Interscience, NY(1954), 71 2 9)Organic Analysis, Interscience. NY, yols 1,2,3 Sr 4-net found Addnl Refs a)B. 1.Ardashev, ZhPriklKhim 10, 1032-41X1041 in Fr)(i 937) CA 32, 1612(1938) RusP 52893(1938) CA 34, 5378(1948)(Detn of carbazole by titrating its AcOH soln with bromide-bromate reagent until the blue coloration of I-starch paper remains for lOmins) c)M.Tl inskii R.B.Roshal, DoklAkadN 17, 117-20(1937)(in... 

Although consumption of the hydroperoxide is normally complete, the absence of this peroxide in the reaction mixture should be established by testing with moist starch-iodide paper or by iodometric titration.2 The amine oxide content may be determined by titration with standard aqueous hydrochloric acid after any amine present has been consumed by reaction with methyl iodide for 1 hour at room temperature.3 From this volumetric analysis the submitters determined the yield of amine oxide to be 86%. The checkers found that the reaction could be followed by measuring the n.m.r. spectra in -butyl alcohol solution where the n.m.r. N-methyl signals of the amine (at S 2.03) and the amine oxide (at S 2.98) are readily observed. 

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