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Fermentation mixture

Isolation. Isolation procedures rely primarily on solubiHty, adsorption, and ionic characteristics of the P-lactam antibiotic to separate it from the large number of other components present in the fermentation mixture. The penicillins ate monobasic catboxyHc acids which lend themselves to solvent extraction techniques (154). Pencillin V, because of its improved acid stabiHty over other penicillins, can be precipitated dkecdy from broth filtrates by addition of dilute sulfuric acid (154,156). The separation process for cephalosporin C is more complex because the amphoteric nature of cephalosporin C precludes dkect extraction into organic solvents. This antibiotic is isolated through the use of a combination of ion-exchange and precipitation procedures (157). The use of neutral, macroporous resins such as XAD-2 or XAD-4, allows for a more rapid elimination of impurities in the initial steps of the isolation (158). The isolation procedure for cephamycin C also involves a series of ion exchange treatments (103). [Pg.31]

The fermentation mixtures from the four fermentors are combined and filtered with the... [Pg.1576]

A simplified scheme is shown in Figure 5.10. The fermentation mixture is filtered to remove mycelium and suspended solids. The solution is treated with hot carbon and then filtered. During the process addification is necessary to reverse the neutralisation by caldum hydroxide or ammonium hydroxide employed during fermentation. [Pg.140]

As early as 1908, Rosenthaler found in the ferment mixture of emulsin a u-oxynitrilase , which directed the addition of hydrocyanic acid (hydrogen cyanide) to benzaldehyde asymmetrically to give x-hydroxybenzeneacetonitrilc (mandelonitrile)9. This result was confirmed1 °, however, it was not until 1963 that Pfeil ct al. first isolated and characterized the enzyme (R)-oxyni-trilase [EC 4.1.2.101 from bitter almonds (Prunus amygdalus)1 12. The yellow-colored enzyme contains a flavin-adenine dinucleotide (FAD)11 and loses its activity by splitting off this prosthet-... [Pg.667]

Air is excluded from the fermentation mixture (anaerobic conditions) to prevent the oxidation of ethanol to ethanoic acid. The process of fractional distillation is then used to obtain a more concentrated solution of ethanol. [Pg.102]

The action of zymase is inhibited once the percentage of alcohol formed exceeds 14 percent. If air gets into fermentation mixture, the ojQTgen of air oxidises ethanol to ethanoic acid which in turn destroys the taste of alcoholic drinks. [Pg.67]

It is worthy of notice that acetaldehyde, a characteristic product of simultaneous oxidation in the fermentation mixture, was detected in these experiments. Its appearance is undoubtedly connected with the process of reduction. Acetaldehyde, which is formed in the absence of air and cannot therefore be a result of secondary oxidation, occurs in approximately constant ratio to the equivalent amount of the methylheptenol. An obvious relationship thus exists between the hydrogenation and the formation of the acetaldehyde. Presumably a disturbance of the normally correlated processes takes place, in the sense of the theory of fermentation. Whereas acetaldehyde normally is reduced to ethyl alcohol, in this case the intermediary acetaldehyde and the added ketone compete for the hydrogen. It thus follows that at least in the initial stage the amount of acetaldehyde which is not reduced must equal the amount that is displaced as a result of interference by the ketone. [Pg.82]

The first experiments made by Neuberg and Nord with the simplest diketone, diacetyl, showed at once that this substance can be hydrogenated phytochemically with comparitive ease. Acetylmethylcarbinol appears as an intermediate (see below), and the end product of reduction is asymmetric and levorotatory. Reduction was effected by the action of fermenting yeast on diacetyl. The 2,3-butanediol that is formed can be isolated by alcohol-ether extraction of the fermentation mixture after concentration in the Faust-Heim apparatus or by steam distillation in an atmosphere of carbon dioxide under ordinary pressure it is then carefully concentrated with the birectifier and obtained in the pure state by final fractionation. [Pg.86]

The formation of furoin from furil represents the simplest and quickest demonstration of phytochemicai reduction for lecture purposes. The presence of the acyloin can be demonstrated, even in extreme dilution, by its characteristic reaction with aqueous or alcoholic sodium hydroxide (deep blue-green color with deep violet-red dichroic irridescence). This reaction can be carried out in unfiltered fermentation mixtures it gives positive results after thirty seconds. The addition of a few crystals of commercially available furil dissolved in one cc. alcohol to a fermenting sugar solution is suflBcient for demonstration. ... [Pg.91]

The hydrolytic reaction would not have been of interest, while the reductive formation would bring the process in line with other analogous phytochemical reductions. The fact that the presence of sodium sulfite in the fermentation mixture has been established, while sulfate is absent, proves that a true hydrogenation does take place. Previously, it was not considered that two possibilities exist for this reaction. It has been ascertained analytically that the precipitate produced by the fermenta-... [Pg.96]

The experimental procedure was essentially the same for all substances mentioned, namely, the method used in the case of nitrobenzene. Some complication arises, however, due to the fact that the solids do not disperse as easily in the fermentation mixture. Although the materials were always added in alcoholic solution to the yeast preparation, partial precipitation was unavoidable. With 21.4 g. of nitrosobenzene a yield of 4 g. of aniline was obtained addition of the same quantity of phenylhydroxylamine gave 7.5 g. of aniline. In both cases azobenzene appeared as a by-product and could be isolated in the pure state. Its formation can be readily explained by a condensation of the intermediary nitrosobenzene with the end product aniline. [Pg.98]

An example of a highly specifie affinity adsorbant of this type is where a monoclonal antibody to a particultu compound is immobilised as shown in Figure 15.14. For instance, a gel with a monoclonal antibody to p-interferon attached has been used in industrial scale extraction of the compound from fermentation mixtures. [Pg.326]

Production of lactobionic acid from lactose through bacterial oxidation is of some interest because of the properties of this substance. Lockwood and Stodola (1950), using/ graveolens, recovered lactobionic acid in 77% yield from a fermentation mixture containing the following per-liter 96 g of anhydrous lactose, 0.62 g of KH2P04, 0.25 g... [Pg.714]

A titre of ca. 20 pg/ml, determined by microbiological assay on agar plates seeded with Candida albicans, was reached in 5 days. The fermentation was stopped. The fermentation mixture was extracted twice with 1 v/v of n-butanol. The combined butanol extracts were washed with 1 v/v of water, dried with anhydrous sodium sulfate and evaporated to dryness under reduced pressure to yield a residue. The oily residue was extracted 3 times with 2 L of methanol. The combined methanol extracts were passed through diatomaceous earth (Celite) and evaporated to dryness to yield an oily residue containing crude Rapamycin. [Pg.3045]

The fermentation mixtures from the four fermenters are combined and filtered with the aid of diatomaceous earth, A material such as Celite 545 can be used. The filtrate is concentrated under reduced pressure to a volume of 10 liters, and the concentrate is treated with 200 grams of activated charcoal (for example, Darco G-60), stirred at room temperature for one hour, and filtered. The charcoal cake is washed with 7.5 liters of water, and then extracted with three 10-liter portions of 50% aqueous acetone. The three aqueous acetone extracts are combined, concentrated under reduced pressure to approximately one liter, and chilled at 5°C for 48 hours. The solid 9-( 5-D-arabinofuranosyljadenine that precipitates is isolated and purified by successive crystallizations from boiling methanol and from boiling water MP 262° to 263°C. [Pg.3425]

This if the continuous process involving evaporation of a liquid into its vapour by heating the liquid. This is followed by cooling and so condensing the vapour back to the liquid. It is used to extract a pure sample of a liquid (or solvent) from an impure mixture, e.g pure water from dirty water or making pure alcohol from a fermented mixture. [Pg.243]

The reaction is catalyzed by the enzymes found in yeast. This reaction can proceed only until the alcohol content reaches about 13% (the percentage found in most wines), at which point the yeast can no longer survive. Beverages with higher alcohol content are made by distilling the fermentation mixture. [Pg.1028]

Starting raw materials (plant extracts, extracts of animal origin, fermentation mixtures). [Pg.544]


See other pages where Fermentation mixture is mentioned: [Pg.409]    [Pg.21]    [Pg.83]    [Pg.2063]    [Pg.1062]    [Pg.1576]    [Pg.145]    [Pg.360]    [Pg.80]    [Pg.379]    [Pg.440]    [Pg.157]    [Pg.78]    [Pg.80]    [Pg.87]    [Pg.93]    [Pg.485]    [Pg.83]    [Pg.607]    [Pg.239]    [Pg.128]    [Pg.405]    [Pg.477]    [Pg.2406]    [Pg.3425]    [Pg.409]    [Pg.237]    [Pg.64]    [Pg.54]    [Pg.55]    [Pg.251]    [Pg.1821]    [Pg.1547]   
See also in sourсe #XX -- [ Pg.247 ]




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