Ion-exchange chromatography paper

Paper chromatography Ion exchange chromatography Electrophoresis Gas chromatography Adsorption chromatography Gel permeation chromatography... 

Radiochemical versions of many other techniques were soon developed, for example, paper chromatography, ion-exchange chromatography. 

Partition chromatography Ion exchange chromatography Paper chromatography Open-column chromatography High-performance liquid chromatography... 

The use of isotopes in biochemistry, particularly radioisotopes, took off after World War II. Developments in electronics and nuclear energy, and the construction of piles in the U.S. and the U.K., enormously improved the production and detection of radioisotopes. At the same time the introduction of paper and ion-exchange chromatography (Chapter 10) revolutionized analytical methods for the separation of low molecular weight compounds, enabling intermediates to be separated rapidly, identified, and estimated. By 1945 strategies for the evaluation of metabolic pathways and cycles were familiar, thanks to the work of Krebs and the pre-war German schools. 

Lederer, M, Chromatographie on Paper Impregnated Ion Exchange Resins. A Preliminary Report. Anal. Chim. Acta 12, 142 (1955). 

See liquid chromatography gas chromatography paper chromatography thin-layer chromatography ion-exchange chromatography gel filtration. 

Purines and pyrimidines are components of nucleic acids and are known to occur in soils. According to Bremner (1967), the nitrogen in purines and pyrimidines accounts for less than 1% of the total soil nitrogen. Much of the earlier information on the presence of these compounds comes from G. Anderson (1957, 1958, 1961) who identified guanine, adenine, cytosine, thymine, and traces of uracil in acid hydrolyzates of humic acids extracted from three Scottish soils. Anderson used paper and ion-exchange chromatography to detect and estimate the concentrations of nucleic acid bases. More... 

Chromatography and electrophoresis Paper chromatography Thin-layer chromatography Gas chromatography Liquid chromatography Ion-exchange chromatography... 

Modem ion chromatography was first reported in 1975 in a landmark paper by Small, Stevens, and Bauman. At present, there are two main types of ion chromatography suppressed ion chromatography and non-suppressed ion chromatography. Ion exchange remains the primary separation mode used in ion chromatography today, although the apparatus used for the separation of the... 

The identification of the reaction products(aminoethylcysteine, cysteamine, hypoyaurine and taurine)has been performed either by paper or ion exchange chromatography, the latter allowed their quantitative estimation(21) These results suggested a purification of the enzyme. 

For the more advanced student, we have extended the section on Quantitative Semi-micro Analysis, and we have included a section dealing with Special Techniques in Separation and Purification, namely Adsorption Chromatography, Paper Chromatography, and Ion- Exchange Processes. 

Acetaldehyde can be isolated and identified by the characteristic melting points of the crystalline compounds formed with hydrazines, semicarbazides, etc these derivatives of aldehydes can be separated by paper and column chromatography (104,113). Acetaldehyde has been separated quantitatively from other carbonyl compounds on an ion-exchange resin in the bisulfite form the aldehyde is then eluted from the column with a solution of sodium chloride (114). In larger quantities, acetaldehyde may be isolated by passing the vapor into ether, then saturating with dry ammonia acetaldehyde—ammonia crystallizes from the solution. Reactions with bisulfite, hydrazines, oximes, semicarb azides, and 5,5-dimethyl-1,3-cyclohexanedione [126-81 -8] (dimedone) have also been used to isolate acetaldehyde from various solutions. 

Finally, the techniques of nmr, infrared spectroscopy, and thin-layer chromatography also can be used to assay maleic anhydride (172). The individual anhydrides may be analyzed by gas chromatography (173,174). The isomeric acids can be determined by polarography (175), thermal analysis (176), paper and thin-layer chromatographies (177), and nonaqueous titrations with an alkaU (178). Maleic and fumaric acids may be separated by both gel filtration (179) and ion-exchange techniques (180). 

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