P-neurexin

Neuroligin is a protein that binds to the PDZs of PSD95. Neuroligin reaches into the synaptic cleft and interacts with [3-neurexin, a protein that reaches into the synaptic cleft from the presynaptic side P-neurexin is anchored presynaptically to the PDZ domain of another MAGUK, CASK. This arrangement connects the pre-and postsynaptic elements and stabilizes the synapse mechanically. 

Boucard, AA, Chubykin, AA, Comoletti, D, Taylor, P and Sudhof, TC (2005) A splice-code for trans-synaptic cell adhesion mediated by binding of neuroligin 1 to a- and P-neurexins. Neuron 48(2) 229-236. 

Krasnoperov VG, Beavis R, Chepumy OG et al (1996) The calcium-independent receptor of a-latrotoxin is not a neurexin. Biochem Biophys Res Commun 227 868-75 Krasnoperov VG, Bittner MA, Beavis R et al (1997) a-Latrotoxin stimulates exocytosis by the interaction with a neuronal G-protein-coupled receptor. Neuron 18 925-37 Krasnoperov VG, Bittner MA, Mo W et al (2002b) Protein tyrosine phosphatase-G is a novel member of the functional family of a-latrotoxin receptors. J Biol Chem 277 35887-95 Kreienkamp HJ, Zitzer H, Gundelfinger ED et al (2000) The calcium-independent receptor for a-latrotoxin from human and rodent brains interacts with members of the ProSAP/SSTRIP/Shank family of multidomain proteins. J Biol Chem 275 32387-90 Lajus S, Lang J (2006) Splice variant 3, but not 2 of receptor protein-tyrosine phosphatase a can mediate stimulation of insulin-secretion by a-latrotoxin. J Cell Biochem 98 1552-9 Lajus S, Vacher P, Huber D et al (2006) a-Latrotoxin induces exocytosis by inhibition of voltage-dependent K+ channels and by stimulation of L-type Ca2+ channels via latrophilin in [5-cells. J Biol Chem 281 5522-31... 

Arac, D, Boucard, AA, Ozkan, E, Strop, P, Newell, E, Sudhof, TC and Brunger, AT (2007) Structures of neuroUgin-l and the neuroUgin-l/neurexin-l beta complex reveal specific protein-protein and protein-Ca2+ interactions. Neuron 56 992-1003. 

Comoletti, D, Elynn, RE, Boucard, AA, Demeler, B, Schirf, V, Shi, J, Jennings, LL, Newlin, HR, Sudhof, TC and Taylor, P (2006) Gene selection, alternative splicing, and post-translational processing regulate neuroligin selectivity for beta-neurexins. Biochemistry 45 12816-12827. 

Dean, C, Scholl, EG, Choih, J, DeMaria, S, Berger, J, Isacoff, E and Scheiffele, P (2003) Neurexin mediates the assembly of presynaptic terminals. Nat Neurosci 6 708-716. 

Fabrichny, IP, Leone, P, Sulzenbacher, G, Comoletti, D, Miller, MT, Taylor, P, Bourne, Y and Marchot, P (2007) Structural analysis of the synaptic protein neuroligin and its beta-neurexin complex determinants for folding and cell adhesion. Neuron 56 979-991. 

Koehnke, J, Jin, X, Trbovic, N, Katsamba, PS, Brasch, J, Ahlsen, G, Scheiffele, P, Honig, B, Palmer, AG, 3rd, and Shapiro, L (2008) Crystal structures of beta-neurexin 1 and beta-neurexin 2 ectodomains and dynamics of splice insertion sequence 4. Structure 16 410-421. 

Taniguchi, H, GoUan, L, Scholl, FG, Mahadomrongkul, V, Dobler, E, Limthong, N, Peck, M, Aoki, C and Scheiffele, P (2007) Silencing of neuroUgin function by postsynaptic neurexins. J Neurosci 27 2815-2824. 

Figure 32.3. Membrane association of neurexin in 6-12-hour embryos. On a 7% SDS-polyacrylamide gel, 10 ig of total protein for lanes T (total extract) and M (membrane extract) was loaded. Approximately 3 pg of membrane protein is used in each membrane-treatment experiment. For the membrane-washing experiment (Protocol 32.2, Method 1), 21 pi of supernatant (S) and 4.2 pi of peUet (P) from PBS (pH 10), and NP-40 washes were loaded in parallel. For the phase-separation experiment (Protocol 32.2, Method 2), 10.5 pi of the Triton suspension (X), 21 pi of the aqueous phase (A), and 21 pi of detergent phase (D) were loaded. Western-blot analysis was performed using rabbit anti-neurexin serum (1 1000 dilution), according to the manufacturer s protocol for ECL (Amersham).

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