P-glycerophosphate

In a commercially available assay, serum NTP catalyzes the hydrolysis of IMP to yield inosine, which is then converted to hypoxanthine by purine-nucleoside phosphorylase (EC 2.4.2.1). Hypoxanthine is oxidized to urate with xanthine oxidase (EC 1.2.3.2). Two moles of hydrogen peroxide are produced for each mole of hypoxanthine liberated and converted to uric acid. The formation rate of hydrogen peroxide is monitored by a spectrophotometer at 510nm by the oxidation of a chromogenic system. The effect of ALPs on IMP is inhibited by p-glycerophosphate. This material is substrate for ALP but not for NTP, and by forming substrate complexes with the former enzyme, it reduces the proportion of the total ALP activity that is directed to the hydrolysis of the NTP substrate, IMP. ... 

Mineralization medium for Saos-2 cells. Add 50 pg/mL ascorbic acid (Sigma) and 7.5 mM p-glycerophosphate (Sigma) in Saos-2 cell culture growth medium (reeNote 3). 

Fig. 2 Inhibition of matrix mineralization. Alizarin Red-staining (A570 nm) of 21 day cultures of WT VSMC in the presence of p-glycerophosphate. The cells were cultured with 30 pmol/L of the TNAP inhibitor MLS-0038949 and the PH0SPH01 inhibitors MLS-0263839 and MLS-0390838, either alone or combined, as indicated. Calcium deposition in 21 -day VSMC cultures was evaluated by staining cell layers with Alizarin Red...

Next day, add mineralizing media, 10 % FCS aMEM containing 10 mM P glycerophosphate-100 pg/ml ascorbic acid and 30 pM of freshly dissolved PHOSPHOl inhibitors in DMSO (PGP, AsA Stocks stored in -20 °C) and renew the mineralizing media every second day till desired point (Day 15-21). Controls contain DMSO only. 

Other chromogenic substrates can also be exploited, including phenolphthalein monophosphate, thymophthalein monophosphate, P glycerophosphate, and uridine phosphate. Fluorigenic substrates can also be used such as P-naphthyl phosphate, 4-methylumbelliferyl phosphate, and 3-o-methylfluorescein monophosphate (21). 

Disodium-p-glycerophosphate [819-83-0 (4H2O), 13408-09-8 (5H2O)] M 216.0, m 102-104 , pKf (free acid). Crystallise it from water. [Attwood et al. Biochem J253 389 1988, Beilstein 1IV 2766.]... 

Apomyoglobin from equine skeletal muscle (Sigma) for modification by HNE (see the section Reagents and Standards ), or similar protein commercially available or isolated Lysis buffer for mammalian cells 20 mM TrisHCl/ 50 mM NaCl/6 M urea (pH 8.1)710 mM sodium pyrophosphate/1 mM sodium fluoride/1 mM P-glycerophosphate/1 mM sodium orthovanadate/1... 

These observations provide convincing evidence that adenosine is continuously formed by the hepatocytes, and has to be rephos-phorylated in order to maintain the adenine nucleotide pool. The results depicted in Fig. 2 were only slightly modified when a mixture of AOPCP and p-glycerophosphate was added to the cell suspensions in order to inhibit the membranous 5 -nucleotidase and aspe-cific phosphatases. This rules out a significant participation of the former enzyme, as well as of the extracellular catabolism of adenine nucleotides released by dammaged hepatocytes, in the formation of adenosine. The production of adenosine thus most likely results from dephosphorylation of AMP inside the cells, by the cytoplasmic 5 -nucleotidase. From the observation that this formation of adenosine does not contribute to the physiological production of allantoin, it can be concluded that both the formation and utilization of adenosine proceed at the same rate in control conditions, and thus constitute a "futile cycle. From... 

Hydrogels Chitosan/p-glycerophosphate hydrogel loaded with anti cancer agents against breast cancer/cervical cjmcer... 

HL-60 cells were lysed with buffer containing 10% glycerol, 1% Triton X-100, 1 mM sodium orthovanadate, 1 mM EGTA, 5 mM EDTA, 10 mM NaF, 1 mM sodium pyrophosphate, 20 mM Tris-HCl pH 7.9, 100 pM P-glycerophosphate, 137 mM NaCl, 1 mM phenylmethylsulfonyl fluoride (PMSF), 10 pg/mL aprotinin and 10 pg/mL leupeptin. The supernatant thus obtained was used for inununoblotting of PARP and DFF45/ICAD and for caspase activity assay. 

Chitosan solutions that are physically mixed with P-glycerophosphate can be injected into the body in liquid form, forming a gel in situ at the body temperature. The rate of gelation depends on the degree of chitosan deacetylation, the concentration of p-glycerophosphate, and the temperature and pH of the final solution [134]. The in situ gelation mechanism involves neutralization of the ammonium... 

Langenbach, F., Handschel, J., 2013. Effects of dexamethasone, ascorbic acid and p-glycerophosphate on the osteogenic differentiation of stem cells in vitro. Stem Cell Research Therapy 4,117. 

Buffer solutions. Buffer A 20 mM Tris (pH 7.5), 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% (v/v) Triton X, 2.5 mM sodium pyrophosphate, 1 mM p-glycerophosphate. Complete protease inhibitor cocktail (Roche Applied Science), phosphatase inhibitor cocktail 1 (Sigma-Aldrich), phosphatase inhibitor cocktail 2 (Sigma-Aldrich) and 20 nM microcystin LR (Calbiochem). Buffer B 25 mM Tris (pH 7.5), 10 mM magnesium chloride, 5 mM p-glycerophosphate, 0.1 mM sodium orthovanadate and 2 mM DTT. 

Ding, K., Yang, Z., Zhang, Y.L., Xu, J.Z., 2013. Injectable thermosensitive chitosan/p-glycerophosphate/collagen hydrogel maintains the plasticity of skeletal muscle satellite cells and supports their in vivo viabUity. Cell Biol. Int. 37 (9), 977-987. 

Niranjan, R., et al., 2013. A novel injectable temperature-sensitive zinc doped chitosan/p-glycerophosphate hydrogel for bone tissue engineering. Int. J. Biol. Macromol. 54, 24—29. Available at http //www.ncbi.nlm.nih.gov/pubmed/23201776 (accessed 02.11.15). 

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