P388 cells

P388 cells implanted i.p., i.p. or i.v. treatment (15 mice per group) N3P3AZ6 was dissolved in 0.9% NaCl solution N. P Azg and N P PyrrOg were suspended in Klucel JF (Hercules Co.) water solution median survival time of control 9.9 days. 

P388 cells implanted i.p.,i.p.treatment(10mice/group) median survival timeof control lO.Odays a This ILS figure was actually vitiated by the mortality of 4 mice out of 10 which were not included in the calculation of %ILS. 

Nakamura S, Wakusawa S, Tajima K, Miyamoto K, Hagiwara M, Hidaka H (1993) Effects of isoquinolinesulphonamide compounds on multidrug-resistant P388 cells. J Pharm Pharmacol 45 268-273... 

In 1999 Uemuraetal. isolatedattenol A (100) and B (101) (Fig. 1.2.5), both marine natural products exhibiting a moderate cytotoxicity against P388 cells [57], from the Chinese Pinna attenuata. Since they are isomeric triols they differ only in the hydroxyl groups involved in the formation of the ketal functionality, so that this results in a l,6-dioxaspiro[4.5]decane and a 6,8-dioxabicyclo[3.2.1]octane unit as the main structural feature of attenol A and B, respectively. 

D. auricularia is a shell-less mollusk that, therefore, initially appears to lack defenses against predators however, this is only a preliminary supposition. Accumulated evidence supports the fact that Opisthobran-chia mollusks have developed very powerful chemical defenses (Pettit et ah, 1989). Pettit et al. (1981) were the first to isolate some of these compounds the pentapeptide dolastatin 10 (Fig. 5.1) was reported to be the most active natural anticancer substance at that time with an ED50 of 4.6x 10 5pg/mL against the P388 cell line (Pettit et al., 2008). Dolastatin 10 was also shown to inhibit tubulin polymerization and tubulin-dependent GTP hydrolysis (Bai et al., 1990). 

White sponge, Dysidea sp. (Fig. 10.5), has been observed to overgrow members of the family Poritidae at Okinawa Island. Extracts from this sponge have been shown to contain brominated diphenyl ethers, which show potent cytotoxicity against P388 cells. Two known compounds, S -dibromo -p /l -dibromophenoxy) and 3,6-dibromo-2-(4 -dibromo-phenoxy)phenol (Fu and Schmitz, 1996 Fu et al., 1995 Fig. 10.6), were... 

Toxoid Microtubule Disassembly Inhibitory Activity1 IC /IC (paclitaxel) P388 Cell Line (Hg/mL) P388/Doxc ( ig/mL)... 

Taxoids Microtubule Disassembly Inhibitory Activity1 ICsq/ICso (paclitaxel) P388 Cell Lineb ICsobiM) P388/Doxb IC50 (pM)... 

Fig. 7.4 (a) Dose-response of the lamellarin M-induced mitochondrial depolarization in P388 cells, (b) Monitoring of the mitochondrial membrane potential (A rm) by realtime flow cytometry, using functional mitochondria isolated from P388 cells and the fluorescent probe JC-1. 

Fig. 7.5 Transmission electron microscopic images of a mitochondrion in P388 cells (a) control, (b) after treatment for 15 min with 5 pM lamellarin D. Magnification, x20000.

Fagaronine Cytotoxic to KB cells, leukemia L12I0, P388 cells — 88,286... 

Nitidine Antileukemic to mouse, L1210, P388 cells Antitrypanosomal — 300... 

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